Ester-Producing Mechanism of Ethanol O-acyltransferase Gene in from Shanxi Aged Vinegar.

The ethanol O-acyltransferase is an important element of key signaling pathways and is widely expressed in yeast strains. In this study, we investigated the expression of 1 in the overexpression lines or knockout system of using qRT-PCR and western blotting. The amount of total protein was determined using the Bradford method; the esterase activity was determined using p-nitrophenyl acetate as a substrate, and the production of volatile fatty acids in wild-type, knockout, and over-expression systems was detected using SPME GC-MS.

Effect of on Postharvest Diseases and Induced Resistance of Strawberry Fruits.

This study takes strawberry-fruits as the test material and discusses the effect of B-1 on preventing postharvest diseases and inducing resistance-related substances in strawberry-fruits. Soaking and wound inoculating is performed to analyze the inhibitory effects of different treatment solutions on the gray mold of postharvest strawberry-fruits. The count of antagonistic bacteria colonies in the wound is found, and the dynamic growth of antagonistic bacteria and the pathogenic fungus is observed by electron microscopy.

[Separation, purification and antioxidant activity of polysaccharide from Coprinus comatus].

We compared the ways of deproteinization for crude polysaccharides of Coprinus comatus, and finally selected Sevage method as the optimal method. Two main fractions of Ccp-I-A and Ccp-I-B were obtained after DEAE-52 cellulose and Sephadex G-200 chromatography, both were white-floc, soluble in water, insoluble in absolute ethyl alcohol, acetone and other organic solvents. Additionally, Fehling reagent, CTAB, Sulphuric acid-carbazole, I-KI and FeCl₃ reaction were all negative.