Publications by authors named "Rudolf B L Brinkman"
Article Synopsis
- CRISPR-Cas9 tools have transformed research in both eukaryotic and prokaryotic systems, but existing Cas9 enzymes struggle with stability at high temperatures.
- The study identifies ThermoCas9 from Geobacillus thermodenitrificans, which is effective at temperatures ranging from 20 to 70 °C and exhibits unique characteristics like PAM-preference and mismatch tolerance.
- ThermoCas9 has been successfully applied for gene engineering and transcriptional silencing in Bacillus smithii and Pseudomonas putida at higher temperatures, expanding the potential for genetic modifications in varying environments.
