Optimal Cell Length for Exploration and Exploitation in Chemotactic Planktonic Bacteria.

Elongated morphologies are prevalent among motile bacterioplankton in aquatic systems. This is often attributed to enhanced chemotactic ability, but how long is best? We hypothesized the existence of an optimal cell length for efficient chemotaxis resulting from shape-imposed physical constraints acting on the trade-off between rapid exploration versus efficient exploitation of nutrient sources. To test this hypothesis, we evaluated the chemotactic performance of elongated cephalexin-treated Escherichia coli towards α-methyl-aspartate in a microfluidic device creating linear, stable and quiescent chemical gradients.

Estimation of sinking velocity using free-falling dynamically scaled models: Foraminifera as a test case.

The velocity of settling particles is an important determinant of distribution in extinct and extant species with passive dispersal mechanisms, such as plants, corals and phytoplankton. Here, we adapted dynamic scaling, borrowed from engineering, to determine settling velocity. Dynamic scaling leverages physical models with relevant dimensionless numbers matched to achieve similar dynamics to the original object.

Motile curved bacteria are Pareto-optimal.

Curved rods are a ubiquitous bacterial phenotype, but the fundamental question of why they are shaped this way remains unanswered. Through in silico experiments, we assessed freely swimming straight- and curved-rod bacteria of a wide diversity of equal-volume shapes parameterized by elongation and curvature, and predicted their performances in tasks likely to strongly influence overall fitness. Performance trade-offs between these tasks lead to a variety of shapes that are Pareto-optimal, including coccoids, all straight rods, and a range of curvatures.

Simulated sunlight action spectra for inactivation of MS2 and PRD1 bacteriophages in clear water.

Action spectra for simulated sunlight were measured in clear water for two viruses: PRD1, a double-stranded DNA bacteriophage, and MS2, a single-stranded RNA bacteriophage. Viruses were diluted into phosphate buffered saline (20 mM PBS, pH 7.5) and exposed for 22 h to simulated sunlight either directly or through one of six glass filters with 50% cutoff wavelengths ranging from 280 to 350 nm.