Photoplethysmography-Based Angiography of Skin Tumors in Arbitrary Areas of Human Body.

Noninvasive, rapid, and robust diagnostic techniques for clinical screening of tumors located in arbitrary areas of the human body are in demand. To address this challenge, we analyzed the feasibility of photoplethysmography-based angiography for assessing vascular structures within malignant and benign tumors. The proposed hardware and software were approved in a clinical study involving 30 patients with tumors located in the legs, torso, arms, and head.

[Alveolar adenoma of the lung].

Alveolar adenoma of the lung is a rare benign tumor first described in 1986. This article presents an observation of alveolar adenoma in a 72-year-old woman. Morphological and immunohistochemical methods of tumor diagnostics, issues of differential diagnosis are analyzed.

Microscopic photoplethysmography-based evaluation of cardiotoxicity in whitefish larvae induced by acute exposure to cadmium and phenol.

Toxic environmental pollutants pose a health risk for both humans and animals. Accumulation of industrial contaminants in freshwater fish may become a significant threat to biodiversity. Comprehensive monitoring of the impact of environmental stressors on fish functional systems is important and use of non-invasive tools that can detect the presence of these toxicants in vivo is desirable.

Artificial intelligence (AI) for tumor microenvironment (TME) and tumor budding (TB) identification in colorectal cancer (CRC) patients: A systematic review.

Evaluation of the parameters such as tumor microenvironment (TME) and tumor budding (TB) is one of the most important steps in colorectal cancer (CRC) diagnosis and cancer development prognosis. In recent years, artificial intelligence (AI) has been successfully used to solve such problems. In this paper, we summarize the latest data on the use of artificial intelligence to predict tumor microenvironment and tumor budding in histological scans of patients with colorectal cancer.

Digital twin concept: Healthcare, education, research.

Introducing the concept of digital twins in healthcare, medical education, and research is a complex multistage challenge requiring participation of multidisciplinary teams. In pursuing this goal, we have created a validated database of scans of colorectal tumor slides associated with relevant clinical and histological information. This database is also linked to the blood bank, which opens a wide range of opportunities for further research.

Complex trigger wrist associated with carpal tunnel syndrome: a case report.

Background: There are few reports of trigger wrist in the literature, as it is a rare pathology. Furthermore, various authors report that it is also hard to diagnose. It manifests with neurological symptoms at the affected wrist, which are usually induced by wrist movement, and can lead to partial or full loss of wrist function and sensitivity.

Spatio-temporal segmentation of image sequences for non-invasive analysis of cardiovascular structure and function in Whitefish embryos.

Label-free quantitative analysis of the cardiovascular structure and function in wild fish is highly important and still unsolved problem. We developed a robust algorithm for bright field microscopy data processing, which allows reliable detection and quantification of circulatory system across the whole animal. We verified this technique by in vivo experiments on broad whitefish (Coregonus nasus) embryos.

Effect of a neurostimulator on postnatal neurogenesis in rodent olfactory bulbs.

The aim of the study was to reveal the effect of neurostimulation with the TKPRPGP neuropeptide on the expression intensity of Doublecortin and Nestin in the olfactory bulb of white Wistar rats using immunohistochemical and computer analysis methods. An isolated assessment of early progenitor differentiation by the density of nestin-positive structures showed that stimulation from birth to 14 days preserves the level of nestin expression, preventing its decrease. When the administration of the neuropeptide is stopped, the expression of nestin decreases sharply, starting from the central zones of the bulb, and after three weeks it is no longer present.

Immunomorphological Features of the Placenta in Allogeneic Pregnancy as the Background for the Development of Obstetric Complications.

Objective: To study the structural and immunohistochemical features of placentas in women after assisted reproductive technology (ART) with allogeneic eggs (oocyte donation and surrogate motherhood).

Study Design: The study involved 89 women whose pregnancy occurred as a result of in vitro fertilization (IVF) with a donor egg in a surrogate motherhood program (IVF-SM, n = 47 patients) or oocyte donation (IVF-DO, n = 42). The comparison group consisted of 21 patients in whom pregnancy occurred as a result of IVF with their own egg (IVF-OE).

[Pathomorphological features of the placentas and the placental sites after in vitro fertilization with a donor egg].

Objective: To identify the structural and immunohistochemical features of the placentas and the placental sites after in vitro fertilization (IVF) with a donor egg (surrogate motherhood).

Subject And Methods: Morphological and immunohistochemical studies were performed on the placental (a placental disk) and placental bed materials obtained after caesarean delivery. The investigation enrolled 26 patients whose pregnancy occurred with IVF with a donor egg according to the surrogacy (IVF-S) program.

6-gene promoter methylation assay is potentially applicable for prostate cancer clinical staging based on urine collection following prostatic massage.

The detection of prostate cancer (PCa) biomarkers in bodily fluids, a process known as liquid biopsy, is a promising approach and particularly beneficial when performed in urine samples due to their maximal non-invasiveness requirement of collection. A number of gene panels proposed for this purpose have allowed discrimination between disease-free prostate and PCa; however, they bear no significant prognostic value. With the purpose to develop a gene panel for PCa diagnosis and prognosis, the methylation status of 17 cancer-associated genes were analyzed in urine cell-free DNA obtained from 31 patients with PCa and 33 control individuals using methylation-specific polymerase chain reaction (MSP).

Bone metabolism genes variation and response to bisphosphonate treatment in women with postmenopausal osteoporosis.

Introduction: Long-term treatment is used in patients with osteoporosis, and bisphosphonates (BPs) are the most commonly prescribed medications. However, in some patients this therapy is not effective, cause different side effects and complications. Unfortunately, at least one year is needed to identify and confirm an ineffectiveness of BPs therapy on bone mineral density (BMD).

Structural, immunohistochemical and molecular features of placentas and placental sites after in vitro fertilization with donor eggs (surrogate motherhood).

Objective: to identify structural, immunohistochemical and molecular features of placentas and placental sites afterin vitro fertilization (IVF) with donor eggs (surrogate motherhood).

Study Design: morphological and immunohistochemical studies were performed on placental material obtained after delivery by caesarean section. The study included 26 women patients whose pregnancy resulted from IVF with a donor egg (IVF-SM group).

[Prediction of activity and severity of endocrine ophthalmopathy by multidimensional linear regression modeling].

Unlabelled: Endocrine ophthalmopathy (EOP) is a severe chronic autoimmune disease associated with autoimmune thyroid pathology that leads to loss of sight, cosmetic defects and quality of life decrease. EOP is difficult to give prognosis for due to various factors affecting its course and outcome.

Purpose: To develop a reliable and precise prognosis method for EOP activity and severity based on personalized combination of risk factors in specific patients by multidimensional linear regression modeling.

Aberrant expression of selenium-containing glutathione peroxidases in clear cell renal cell carcinomas.

Aim: To find putative diagnostic markers for clear cell renal cell carcinomas (ccRCC).

Material And Methods: Quantitative polymerase chain reaction (Q-PCR), bisulfite treatment, methylation-specific PCR, analysis on cBioPortal for Cancer Genomics.

Results: We have found that expression of GPX1, GPX3, and GPX4 genes was decreased in ccRCC.

[Performance of fragmenting operation for restoration of a correct rhythm while treating the isolated failure of a mitral valve].

Possibilities of application of the proposed procedure for intraoperative restoration of the sinus rhythm while the mitral valve prosthesis (MVP) were studied up. In 161 patients, suffering the isolated mitral valve failure stage IV, a surgical treatment was conducted in Department of surgical treatment of acquired heart failures. The left atrium plasty was conducted in 119 (73.

Epigenetic alterations of chromosome 3 revealed by NotI-microarrays in clear cell renal cell carcinoma.

This study aimed to clarify epigenetic and genetic alterations that occur during renal carcinogenesis. The original method includes chromosome 3 specific NotI-microarrays containing 180 NotI-clones associated with 188 genes for hybridization with 23 paired normal/tumor DNA samples of primary clear cell renal cell carcinomas (ccRCC). Twenty-two genes showed methylation and/or deletion in 17-57% of tumors.

Association Between Polymorphisms of VDR, COL1A1, and LCT genes and bone mineral density in Belarusian women with severe postmenopausal osteoporosis.

BACKGROUND AND OBJECTIVE. Variation of osteoporosis in the population is the result of an interaction between the genotype and the environment, and the genetic causes of osteoporosis are being widely investigated. The aim of this study was to analyze the association between the polymorphisms of the vitamin D receptor (VDR), type I collagen (COL1A1), and lactase (LCT) genes and severe postmenopausal osteoporosis as well as bone mineral density (BMD).

[Functional tear production indices in thyroid eye disease].

Analysis of functional tear production indices in patients with Grave's disease and thyroid eye disease confirmed lacrimal gland involvement in inflammatory autoimmune process. Close direct correlation was found between tear production and main disease characteristics - severity and activity.

Congenital emphysema in children: segmental lung resection as an alternative to lobectomy.

Background: Congenital emphysema is a rare lung malformation characterized by overinflation of lung segments or lobe, together with ventilation and lung perfusion disorders. The lesion frequently causes respiratory distress in infants and may require urgent surgery. The pathogenesis is still controversial, and the usual treatment recommended is a lobectomy.

[Morphological changes in autoimmune dacryoadenitis associated with thyroid eye disease].

Increase of lacrimal gland (LG) volume was revealed in 1/3 of patients with thyroid eye disease (TED) using visualization methods. Authors show morphological data confirming development of autoimmune process in LG in TED, biopsy samples were studied within 6 hours after exitus. Specific histomorphological characteristics were analysed considering dystrophic and degenerative changes in LG tissue.

Genetic and epigenetic changes of NKIRAS1 gene in human renal cell carcinomas.

Unlabelled: Renal cell carcinoma (RCC) is the most common malignant tumor of kidney associated with the worst clinical outcome. No molecular markers for RCC diagnostics and prognosis that could be applied in clinics were described yet. Large-scale screening of 3p human chromosome genes/loci in RCC and histologically normal tissues surrounding the tumors using NotI-microarray approach demonstrated that NKIRAS1 gene contained the largest percent of genetic/epigenetic changes in RCC tumor cells.

[Functional state of arterioles in patients with essential hypertension and arterial hypertension developed at the background of diabetes mellitus].

With the aim of studying linear velocity characteristics of arteriolar blood flow and reactivity of arterioles we examined 80 patients with essential arterial hypertension (AH), 83 patients with type 2 diabetes mellitus (DM) with concomitant AH, and 63 healthy people. In all patients examination included ultrasound continuous wave dopplerography of microcirculatory vascular bed at rest and after distant warming test. It was found that in patients with essential AH blood flow velocity in arterioles was maximal, increment of arteriolar blood flow in response to warming was lower than in healthy persons.

[Arteriolar circulation and arterial pressure in patients with essential hypertension].

Arteriolar circulation and its relationship with arterial pressure were studied in 127 patients with essential hypertension and 63 healthy subjects using continuous US dopplerography of the microcirculatory system at rest. Linear arteriolar blood flow rate was measured in healthy subjects and 47 patients before and after primary indapamide therapy. It was found to equal 17.