Promoter Proximal Pausing Limits Tumorous Growth Induced by the Yki Transcription Factor in .

Promoter proximal pausing (PPP) of RNA polymerase II has emerged as a crucial rate-limiting step in the regulation of gene expression. Regulation of PPP is brought about by complexes 7SK snRNP, P-TEFb (Cdk9/cycT), and the negative elongation factor (NELF), which are highly conserved from to humans. Here, we show that RNAi-mediated depletion of or of the 7SK snRNP complex or depletion of individual components of the NELF complex enhances Yki-driven growth, leading to neoplastic transformation of wing imaginal discs.