Recent advancements in soluble expression of recombinant antibody fragments in microbial host systems.

Recombinant fabs dominate the pharmaceutical pipelines today with microbial host systems continuing to be a major contributor toward their production. is a versatile host for recombinant protein expression due to its simplicity, affordability, and ability to be cultivated at high cell density. It is particularly suitable for non-glycosylated proteins and small proteins.

Intensification of delignification of sawdust and subsequent enzymatic hydrolysis using ultrasound.

The current work investigates intensification of delignification of sawdust and subsequent enzymatic hydrolysis to produce reducing sugars with the use of ultrasound. Alkaline hydrolysis of sawdust was initially performed to remove lignin which hampers the rate of enzymatic hydrolysis. Effect of different parameters in the case of ultrasound (US) assisted and conventional processes such as alkali concentration (0.