Publications by authors named "Ruby Rios"
Parasitology
December 2014
Article Synopsis
- The study investigates the clinical accuracy of molecular diagnostic methods for cutaneous leishmaniasis (CL), focusing on different sample sources and DNA extraction techniques used on lesion samples from 105 patients.
- Three DNA extraction methods were compared: Qiagen on swab and aspirate samples, Isohelix on swabs, and Boil/Spin on aspirates, with real-time PCR used to detect Leishmania DNA.
- Results showed that swab sampling combined with Qiagen extraction was the most effective, achieving 98% sensitivity and 84% specificity, making it a simple and effective method for diagnosing CL compared to less sensitive methods using aspirated material.
