Publications by authors named "Ruby M Miller"
Article Synopsis
- The study discusses the effective and selective synthesis of iminosugars, specifically 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) and lentiginosine, focusing on their seven stereoisomers.
- The starting material for these syntheses is 4-benzoyl-6-deoxy-6-iodoglycopyranosides, yielding between 38% to 68% for DAB and between 44% to 89% for lentiginosine.
- A key highlight of the process is a complex multistep reaction involving zinc-mediated reductive elimination, reductive amination, and intramolecular nucleophilic substitution, which allows for the
G protein-coupled receptors (GPCRs) serve critical physiological roles as the most abundant family of receptors. Here, we describe the design of a generalizable and cell lysate-based method that leverages the interaction between an agonist-activated GPCR and a conformation-specific binder to reconstitute split nanoluciferase (NanoLuc) in vitro. This tool, n vitro PCR split anoLuc lgand riggered eporter (IGNiTR), has broad applications.
View Article and Find Full Text PDF