Marginal fit and microbial leakage along the implant-abutment interface of fixed partial prostheses: An in vitro analysis using Checkerboard DNA-DNA hybridization.

Statement Of Problem: Bidirectional leakage through the implant-abutment interface still constitutes a major concern in implant-supported restorations.

Purpose: The purpose of this in vitro study was to evaluate the marginal fit, before and after loading simulation, of 3-unit fixed partial prostheses supported by external hexagon or Morse cone implants and to identify and quantify up to 43 microbial species penetrating through the implant-abutment interface after loading.

Material And Methods: Forty-eight dental implants with external hexagon (EH; n=24) or Morse cone (MC; n=24) connections were investigated.