Fast In Vitro Synthesis and Direct Labeling of Nanobodies for Prototyping in Microscopy Applications.

Small antigen binders, such as nanobodies, have become widely used in biomedical research and pharmaceutical development. However, the pipeline for the generation of functional conjugated probes and drugs from identified binders remains a major time-consuming bottleneck. Here, we developed a method for fast nanobody production and conjugation based on an in vitro synthesis platform.

Synthesis of mono Cytochrome P450 in a modified CHO-CPR cell-free protein production platform.

Cytochromes P450 (CYPs) are a group of monooxygenases that can be found in almost all kinds of organisms. For CYPs to receive electrons from co-substrate NADPH, the activity of NADPH-Cytochrome-P450-oxidoreductase (CPR) is required as well. In humans, CYPs are an integral part of liver-based phase-1 biotransformation, which is essential for the metabolization of multiple xenobiotics and drugs.

Vesicle-based cell-free synthesis of short and long unspecific peroxygenases.

Unspecific peroxygenases (UPOs, EC 1.11.2.