Publications by authors named "Ruben Lachica"
Background: Bladder exstrophy is a rare congenital anomaly affecting the lower abdominal wall, pelvis, and genitourinary structures. Pregnant women with bladder exstrophy present a unique challenge to the obstetrician.
Case: The patient is a 35-year old pregnant woman with bladder exstrophy, an extensive surgical history, and uterine prolapse with an abnormal, rubbery consistency to her cervix.
Diagnosis and Management of Hyperthyroidism in Pregnancy: A Review.
Obstet Gynecol Surv
November 2016
Importance: Hyperthyroidism has important implications for pregnancy, affecting both mother and fetus. Appropriate maternal and fetal management iscritical to avoiding adverse pregnancy outcomes and requires a multidisciplinary approach.
Objective: To describe maternal diagnosis and management of hyperthyroidism, across all stages of pregnancy.
Liposome-mediated delivery of iminosugars enhances efficacy against dengue virus in vivo.
Antimicrob Agents Chemother
December 2012
A key challenge faced by promising antiviral drugs, such as iminosugars, is in vivo delivery to achieve effective levels of drug without toxicity. Four iminosugars, all deoxynojirimycin (DNJ) derivatives-N-butyl DNJ (NB-DNJ), N-nonyl DNJ, N-(9-methoxynonyl) DNJ, and N-(6'-[4″-azido-2″-nitrophenylamino]hexyl)-1-DNJ (NAP-DNJ)-potently inhibited both the percentage of cells infected with dengue virus and release of infectious virus from primary human monocyte-derived macrophages, demonstrating their efficacy in primary cells. In a lethal antibody-dependent enhancement mouse model of dengue pathogenesis, free NB-DNJ significantly enhanced survival and lowered viral load in organs and serum.
View Article and Find Full Text PDFDengue virus (DENV) causes dengue fever and dengue haemorrhagic fever/dengue shock syndrome, both considered major public-health problems worldwide. We generated a lethal DENV-2 strain (D220) by 10 additional cycles of subcutaneous inoculation of mice with supernatant from mosquito cells infected with the previously characterized strain D2S10, followed by harvesting of serum. D220 induces mortality at ten-fold lower doses than D2S10 in mice lacking only the alpha/beta interferon (IFN-α/β) receptor in C57BL/6 or 129 backgrounds under both non-enhanced and antibody-enhanced conditions.
View Article and Find Full Text PDFArticle Synopsis
- If someone gets dengue from one virus type and then gets infected with a different type, it can make their sickness worse because of special antibodies in their body that react to both viruses.
- In experiments with mice, researchers discovered that giving them antibodies increased the severity of the dengue disease, showing symptoms similar to those seen in very sick humans.
- They also found that a modified antibody that couldn't attach to a specific receptor helped protect against severe dengue disease, which may lead to better treatments in the future.