[Breeding of transgenic mice expressing human tau isoform with P301L mutation and identification of homozygous transgenic mice].

Objective: To establish homozygous transgenic mouse strain expressing human tau isoform with P301L mutation.

Methods: Five transgenic mice expressing human tau isoform with P301L mutation were obtained by microinjection into male nuclei. Homozygote and hemizygote were identified by PCR and real-time fluorescent quantitative PCR.