Publications by authors named "Ru-xin Zhu"
Traceability of Geographic Origin Using Human Skin and Oral Microbiota.
Fa Yi Xue Za Zhi
December 2023
Objectives: To explore the possibility of using human skin and oral microorganisms to estimate the geographic origin of an individual through the sequencing analysis of bacterial 16S rRNA gene.
Methods: Microbial DNA was extracted from the palm and oral microorganisms of the Han population in Shanghai and Chifeng, Inner Mongolia, and the composition and diversity of the microbiota were analyzed by full-length 16S rRNA gene sequencing. Then, differential species were screened and a geographic location prediction model was constructed.
Objective: To study the forensic application of Goldeneye DNA ID 26Y Kit in the She nationality.
Methods: Through capillary electrophoresis, the genotype of 26 Y-STR loci were analyzed in 53 unrelated male individuals from Fujian She nationality. The population genetics parameters such as allele frequency and haplotype diversity were calculated.
[Linkage disequilibrium and mutation rate analysis of sixteen X-STR loci].
Fa Yi Xue Za Zhi
December 2014
Objective: To assess the patterns of linkage disequilibrium (LD) of 16 STR loci on X chromo- some and investigate the genetic stability.
Methods: Genomic DNA samples extracted from blood stains from 500 unrelated individuals and 885 lineage members from Eastern Chinese Han population were genotyped through multiplex amplification using IDtyperX-16 kit by our independent research followed by capillary electrophoresis. LD was assessed by PowerMarker v3.
Article Synopsis
- This study assesses the effectiveness of 30 insertion/deletion (InDel) loci from the Investigator DIPplex Kit for forensic purposes in the Han and She ethnic groups in Eastern China.
- Researchers analyzed genetic data from 565 Han and 119 She individuals, calculating allele frequencies and other genetic parameters.
- The findings reveal good genetic diversity in both groups, with the loci proving useful for specific paternity cases without any notable genetic deviations.
Article Synopsis
- Forensic DNA typing has evolved through three generations, with short tandem repeats (STR) currently being a standard tool for identification.
- Recent advancements in genomic research have led to the identification of polymorphism markers such as single nucleotide polymorphisms (SNP) and Insertion/Deletion (InDel) markers, which show promise in forensic and medical biology.
- The paper reviews the historical development of InDel research, highlights their application in forensic biology and anthropology, and discusses future research directions and challenges that need to be addressed.
[Forensic application of investigator Argus X-12 kit in Han population from East China].
Fa Yi Xue Za Zhi
October 2011
Objective: To investigate the genetic data of the 12 X-STR included in Investigator Argus X-12 kit and to evaluate the forensic application in Han population from East China. 9: By detecting 309 unrelated individuals with Investigator Argus X-12 kit, allele frequencies, population genetics parameters and the information of linkage disequilibrium of the 12 X-STR were analyzed by statistics and were compared with available data of other Han populations from different regions.
Results: No deviations from Hardy-Weinberg equilibrium were detected.