3D Printing for Personalized Solutions in Cervical Spondylosis.

In the context of the digital revolution, 3D printing technology brings innovation to the personalized treatment of cervical spondylosis, a clinically common degenerative disease that severely impacts the quality of life and increases the economic burden of patients. Although traditional surgeries, medications, and physical therapies are somewhat effective, they often fail` to meet individual needs, thus affecting treatment adherence and outcomes. 3D printing, with its customizability, precision, material diversity, and short production cycles, shows tremendous potential in the treatment of cervical spondylosis.

Distinct expression of chemokine-like factor 1 in synovium of osteoarthritis, rheumatoid arthritis and ankylosing spondylitis.

Chemokine-like factor 1 (CKLF1) is a newly cloned chemotactic cytokine with CCR4 being its functional receptor. Recent evidence demonstrates a role of CKLF1 in arthritis. The aim of this study was to quantify the expression of CKLF1 as well as assess the correlation between CKLF1 and plasma acute-phase markers.

Quercetin sensitizes human glioblastoma cells to temozolomide in vitro via inhibition of Hsp27.

Aim: Quercetin is an effective Hsp27 inhibitor and has been reported to facilitate tumor cell apoptosis. The aim of this study was to investigate whether quercetin could sensitize human glioblastoma cells to temozolomide (TMZ) in vitro.

Methods: Both U251 and U87 human glioblastoma cells were treated with quercetin and/or TMZ for 48 h.

γ-secretase inhibitor DAPT sensitizes t-AUCB-induced apoptosis of human glioblastoma cells in vitro via blocking the p38 MAPK/MAPKAPK2/Hsp27 pathway.

Aim: Trans-4-[4-(3-adamantan-1-yl-ureido)-cyclohexyloxy]-benzoic acid (t-AUCB) is a soluble epoxide hydrolase inhibitor that suppresses glioblastoma cell growth in vitro. The aim of this study was to examine whether the γ-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT) could sensitize glioma cells to t-AUCB-induced apoptosis.

Methods: Both U251 and U87 human glioblastoma cell lines were tested.

Development of clinically relevant orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma through surgical tumor tissues injection with trocar.

Objective: Orthotopic models are important in cancer research. Here we developed orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma with a specially designed system.

Methods: Tiny fragments of surgical tumors were implanted into the mice brain with a trocar system.

[Transplantation and magnetic resonance imaging of transplantable human glioma tissue in the brain of nude mice].

Background & Objective: In previous reports, orthotopic transplantation models of glioma were produced by injecting cell suspension into the brain of mice, which is complex, time-consuming, and nearly impossible to prepare in a large scale within a short period. This study was to establish human glioma orthotopic transplantation model in nude mice by transplanting tumor tissue in the brain, and investigate magnetic resonance imaging (MRI) of the transplanted tumors.

Methods: Human glioma cells were injected subcutaneously into nude mice to form human glioma.