[Liposome-mediated glial growth factor 2 gene therapy in brain injury: an experimental study with rats].

Objective: To explore the protective effect of glial growth factor-2 (GGF2) on brain injury.

Methods: Thirty-four SD rats underwent lateral fluid percussion to establish brain injury models and then were randomly divided into 4 groups: treatment group (n = 10, the plasmid pEGFP-N1-GGF2 mixed with liposome was injected into the brain tissue directly), vector control group (n = 10, the vector pEGFP-N1 mixed with liposome was injected into the brain tissue directly), liposome control group (n = 10, liposome was injected), and sham operation group (n = 4). Three assessment tasks were performed for neurobehavioral evaluation: Clivas Test, Beam Balance Test and Beam Walking Test.

[Establishment of animal model of chronic optic nerve injury and pathological changes thereof: experiment with cats].

Objective: To establish an animal model of chronic optic nerve injury which is suitable for experimental research.

Methods: Dil, a tracer, was injected through the bone windows into the brain of 48 cats so as to mark the retinal ganglion cells (RGCs). Two weeks later the 48 cats were randomly divided into 6 equal groups.