[A method for construction of restriction cDNA library].

A kind of cDNA library constructed according to Restriction Display PCR(RD-PCR) technology setup in our lab,which was called restriction cDNA library,was introduced in this paper. In the construction of cDNA library, cDNA was digested with restriction endonuclease, linked with special adaptor, amplified with PCR in groups. Each group of the restriction cDNA library contained special cDNAs.

[The cloning of human augmentor of liver regeneration-interacting proteins].

Aim: To screen human augmentor of liver regeneration(hALR)-interacting proteins and to explore the mechanisms of hALR in liver regeneration.

Methods: The open reading frame of hALR was used to construct the "bait" plasmid and the genes encoding hALR-interacting proteins were screened from the human liver cDNA library that was pretransformed into yeast Y187 by yeast two-hybrid system. Bioinformatic analysis of the sequences of the positive clones were performed.