[Expression of mouse B7-H3-Fc fusion protein and characterization of its bioactivity].

Aim: To obtain mouse B7-H3-Fc fusion protein and to investigate its biological function and effects on T lymphocyte activation.

Methods: The genes coding extracellular domain of mouse B7-H3 and the Fc fragment of human IgG1 were amplified from pMD19-T/mouse B7-H3 and pMD19-T/human IgG1 vectors by PCR. The two genes were combined with mouse B7-H3-Fc fragment by overlap PCR.

Biologic characteristics of the side population of human small cell lung cancer cell line H446.

Background And Objective: Recently, the theory of cancer stem cells (CSCs) has presented new targets and orientations for tumor therapy. The major difficulties in researching CSCs include their isolation and purification. The aim of this study is to identify and characterize the side population (SP) cells in small cell lung cancer (SCLC) cell line H446, which lays the foundation for the isolation and purification of CSCs.