Publications by authors named "Ru-Qian Yue"

Background: Although the depth of the counting chamber is an important factor influencing sperm counting, no research has yet been reported on the measurement and comparison of the depth of the chamber. We measured the exact depths of six kinds of sperm counting chambers and evaluated their accuracy.

Materials And Methods: In this prospective study, the depths of six kinds of sperm counting chambers for both manual and computer-aided semen analyses, including Makler (n=24), Macro (n=32), Geoffrey (n=34), GoldCyto (n=20), Leja (n=20) and Cell-VU (n=20), were measured with the Filmetrics F20 Spectral Reflectance Thin-Film Measurement System, then the mean depth, the range and the coefficient of variation (CV) of each chamber, and the mean depth, relative deviation and acceptability of each kind of chamber were calculated by the closeness to the nominal value.

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Article Synopsis
  • The study aimed to investigate how the depth of sperm counting chambers affects sperm motility by analyzing various semen samples using specialized measurement systems.
  • Four sperm counting chambers with different depths were tested, revealing that deeper chambers resulted in lower percentages of progressively motile sperm while non-progressively motile sperm increased.
  • The findings suggest that depth should be considered in sperm analysis to avoid misdiagnosis, indicating the need for updated reference ranges based on chamber depth.
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