Genome sequence of a novel endornavirus from the phytopathogenic fungus Alternaria brassicicola.

In an effort to discover new mycoviruses from phytopathogenic fungi, a dsRNA molecule of 10,290 nt, resembling those associated with the viruses belonging to the family Endornaviridae, was isolated from Alternaria brassicicola, one of the causal agents of rapeseed black spot disease. Genome analysis revealed the presence of a single open reading frame coding for a polyprotein of 3400 aa containing conserved viral methyltransferase (MTR), viral RNA helicase 1 (Hel-1), and RNA-dependent RNA polymerase (RdRp) domains. In addition, a cysteine-rich region (CRR) with conserved CXCC motifs, shared among several endornaviruses, was also identified between the MTR and Hel-1 domains.

The complete nucleotide sequence and genomic organization of a novel victorivirus with two non-overlapping ORFs, identified in the plant-pathogenic fungus Phomopsis vexans.

In this study, a novel virus designated Phomopsis vexans RNA virus 1 (PvRV1) was identified in a strain of Phomopsis vexans. The complete genomic nucleotide sequence was determined and analyzed. Sequence analysis indicated that PvRV1 is closely related to viruses in the genus Victorivirus of the family Totiviridae.

[Effects of terminal products on hydrogen production by ethanol hydrogen-producing microflora].

The effects of terminal products on hydrogen production were conducted in continuously stirred tank reactor (CSTR) by the acclimated ethanol hydrogen-producing bacteria. Glucose was added as carbon source and different ambient pH was carried out by buffer solution. High ethanol production was simultaneously achieved with high hydrogen production.