Publications by authors named "Ru-Gang Chen"

Dehydrins (DHNs) play an important role in abiotic stress tolerance in a large number of plants, but very little is known about the function of DHNs in pepper plants. Here, we isolated a YSK-type DHN gene "" from pepper. To authenticate the function of in salt and drought stresses, it was overexpressed in Arabidopsis and silenced in pepper through virus-induced gene silencing (VIGS).

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NAC (NAM, ATAF1/2 and CUC2) proteins are plant-specific transcription factors (TFs) that are important in plant abiotic stress responses. In this study we isolated a NAC gene from Capsicum annuum leaves, designated as CaNAC064. We characterized the amino acid sequence of CaNAC064 and found that it contain conserved domains of the NAC family, including a highly conserved N-terminus domain and a highly variable C-terminus domain.

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Dehydrins play an important role in improving plant resistance to abiotic stresses. In this study, we isolated a dehydrin gene from pepper ( L.) leaves, designated as Sub-cellular localization of was to be found in the nucleus and membrane.

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Dehydrins (DHNs) play a crucial role in enhancing abiotic stress tolerance in plants. Although DHNs have been identified and characterized in many plants, there is little known about Capsicum annuum L., one of the economically important vegetable crops.

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Article Synopsis
  • The study identifies a new NAC transcription factor called CaNAC2 from Capsicum annuum, which is similar to a protein from Petunia and plays a role in plant development and stress regulation.
  • CaNAC2 has a conserved NAC domain at its N-terminus and is mainly expressed in seeds and roots, with its expression increasing in response to cold, salt, and ABA treatments.
  • Silencing CaNAC2 makes pepper seedlings more susceptible to cold stress and hampers leaf chlorophyll degradation under salt stress, suggesting its crucial role in abiotic stress tolerance in peppers.
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Article Synopsis
  • Researchers isolated the CaDHN1 gene from pepper (Capsicum annuum L.) and found it significantly upregulated in response to cold, salt, osmotic stresses, and salicylic acid (SA).
  • Dehydrins (DHNs) like CaDHN1 are proteins linked to helping plants cope with environmental stresses, and the gene was characterized as belonging to the SKn subgroup based on its structure.
  • Using a technique called virus-induced gene silencing (VIGS), the study demonstrated that disabling CaDHN1 reduces pepper plants' ability to tolerate various abiotic stresses.
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Article Synopsis
  • Plant aquaporins, like CaPIP1-1, facilitate water transport across cell membranes and are crucial for plant responses to environmental stressors.
  • CaPIP1-1 was identified from a pepper species, showing a specific genetic structure similar to related plants and high expression in fruit, especially under stress conditions.
  • Silencing CaPIP1-1 in pepper plants led to reduced growth and lower tolerance to salt and mannitol stress, indicating its critical role in stress response.
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Article Synopsis
  • * Overexpression of CaTIP1-1 results in larger stomatal pores, which decreases chilling tolerance but improves growth in salt and mannitol conditions.
  • * The gene's expression is strongly induced during salt and mannitol stress, and its silencing leads to reduced tolerance, indicating its crucial role in stress response.
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Background: The pepper fruit is the second most consumed vegetable worldwide. However, low temperature affects the vegetative development and reproduction of the pepper, resulting in economic losses. To identify cold-related genes regulated by abscisic acid (ABA) in pepper seedlings, cDNA representational difference analysis was previously performed using a suppression subtractive hybridization method.

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Low temperature is one of the major factors limiting pepper (Capsicum annuum L.) production during winter and early spring in non-tropical regions. Application of exogenous abscisic acid (ABA) effectively alleviates the symptoms of chilling injury, such as wilting and formation of necrotic lesions on pepper leaves; however, the underlying molecular mechanism is not understood.

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High temperature has already become a noticeable environmental factor for crop production, while plant pollen was the most sensitive organ to high temperature stress. In this paper, the cytological, physiological, and molecular biological studies on the high temperature stress on crop pollen were reviewed, aimed to provide ideas for maintaining high productive ability of crops under high temperature stress. The cytological effects of high temperature on crop pollen included the changes of arrangement patterns of rough endoplasmic reticulum in tapetum cells, the irregularity of vascular bundle sheath cells in connective tissue, and the reduction of vesicle production by dictyosomes of pollen tube, etc.

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