Studies on bcl-2 protein in cultured lymphoid cells.

Bcl-2 protein was analysed in cell cultures derived from non-Hodgkin lymphomas and chronic lymphocytic leukaemia patients. The presence of bcl-2 proto-oncogene protein product has been demonstrated on cytospin preparations utilising immunochemical methods. The correlation between the expression of the bcl-2 protein and the appearance of proliferation- or differentiation related figures was studied.

[Myelodysplastic syndrome associated with clonal proliferation of B lymphocytes].

We present an unusual case of the myelodysplastic syndrome (subtype refractory anemia with the excess of blasts in transformation--RAEB-t) associated with significant increase of IgG (4,700 mg/dl), lambda (160 U/dl) in blood serum and circulating clone of B lymphocytes SIgG, lambda, manifesting clonal rearrangement of JH domain. Peripheral blood cells of the patient showed two different chromosomal abnormalities: 47,XY, + del/8/p? and 47,XY, +22, +14, -19. We suppose that two independent neoplastic clones are developed in the described case, i.

[In vitro studies directed at inducing differentiation of leukemic B-lymphocytes].

Lipopolysaccharide (LPS) and phorbol esters (TPA) stimulate lymphocytes proliferation in two different ways. While LPS primary function is specific receptor binding, TPA directly activate cellular protein kinase C. The stimulation of human leukaemic lymphocytes (from chronic lymphocytic leukaemia patients) with LPS and TPA results in two different types of response: to both stimulators, and to LPS only.

[Immunologic phenotype of lymphocytes in chronic B-cell lymphocytic leukemia. II. Studies of immunologic phenotype of peripheral blood and lymph node lymphocytes in patients with chronic lymphocytic leukemia or leukemic forms of lymphoplasmacytoid lymphoma].

Unlabelled: In a group of 16 patients with chronic lymphocytic leukaemia and leukaemic forms of the lymphoma lymphoplasmocytoides immunophenotypes of peripheral blood and lymph node lymphocytes were studied.

Conclusions: 1) immunophenotype heterogeneity observed in a minority of patients in lymph-nodes or peripheral blood seems to be connected with the co-existence of leukaemic and normal reactive B-cells, 2) SIgG+ cells seem to represent activated B lymphocytes producing and secreting autoantibodies, 3) circulating peripheral T lymphocytes do not reflect the distribution of T cell subpopulations in lymph-nodes.

[Phenotypic changes in peripheral blood lymphocytes of patients with chronic lymphoma and B-cell leukemia after treatment].

The purpose of the study was evaluation of the effect of treatment on the immunological phenotype of peripheral blood lymphocytes in patients with chronic lymphocytic leukaemia or in leukaemic forms of non-Hodgkin lymphomas, with low or medium grade malignancy. The study was carried out in 13 patient. In those responding to treatment a tendency was observed for normalization of the immunological phenotype of peripheral blood lymphocytes (increased proportion of cells forming rosettes with sheep erythrocytes, reduced proportion of cells forming rosettes with murine erythrocytes), and a tendency for polyclonal pattern of B-cells with immunoglobulins on their surface (although this feature was not a rule).

A Polish variant of isolated dentinogenesis imperfecta with a generalised connective tissue defect.

A case is described of four generations of a Polish family with characteristic features of dentinogenesis imperfecta, who also presented with a variety of generalised connective tissue features, suggestive of a structural gene mutation causing abnormalities in connective tissues other than dentine.

Different maturation capabilities of chronic lymphocytic leukaemia lymphocytes in vitro.

Peripheral blood lymphocytes from five patients with B-derived chronic lymphocytic leukaemia were stimulated by Staphylococcus aureus strain Cowan together with T cell mitogen phytohaemagglutinin in 5-9 days suspension cultures. The responses of B lymphocytes were studied on a T cell depleted subpopulation, obtained from harvested lymphocyte cultures using the sheep red blood cell rosette technique. Proliferation tests were performed using a 3H-TdR blast cell index.

Interindividual phenotypic variations in CLL B lymphocyte maturation indication of different maturation blocks.

Peripheral blood lymphocytes from 8 patients with B-derived chronic lymphocytic leukaemia were stimulated by Staphylococcus aureus bacteria strain Cowan 1 with T cell mitogens PHA or PWM in 5-7 day suspension cultures. For the first group of patients proliferation and maturation tests were performed on T cell enriched and T cell depleted subpopulations, obtained from harvested lymphocytes at the end of cultures by the sheep red cell rosette technique. To re-examine mutual influences of cultivated T and B cells in the second group of experiments, lymphocytes from CLL patients and from 5 healthy individuals were investigated by the use of transmembrane cocultivation system after Feldman and Basten.

Increased expression of fragile site Xq by hydroxyurea. Preliminary communication.

The addition of hydroxyurea in final concentration 1.3 X 10(-5) M increases the frequency of fraX in comparison with cultures without hydroxyurea. The same concentration of hydroxyurea did not induce fraX formation and "nonspecific" gaps in cultures of lymphocytes derive from healthy individuals.

Cytogenetic findings in 311 couples with infertility and reproductive disorders.

Chromosome studies in married couples were performed in order to elucidate their infertility, spontaneous abortions and foetal wastage. Peripheral blood lymphocyte metaphases of 311 persons revealed chromosomal abnormalities in 26 married people. Chromosome aberrations found here were distributed among sex chromosome aneuploidy (complete and mosaic form), structural anomalies of the Y chromosome, autosomal chromosome aneuploidy (mosaic form), and balanced autosomal translocations (complete and mosaic forms).

[Mononuclear peripheral blood cells in patients with non-Hodgkin's lymphoma synthesizing DNA in vitro].

In 82 patients with non-Hodgkin lymphoma (NHC) the DNA synthesis by mononuclear cells from the peripheral blood was assessed by means of the index of mitoses (IM) or by pulse labelling of cells with 3H-TdR. In chronic lymphatic leukaemia (47 cases), hairy-cell leukemia (1 case), plasma-cell leukaemia (1 case) no synthesis of DNA was found in mononuclear cells. On the other hand, it was raised in most cases of lymphoplasmocytoma, centrocytoma, centroblasto-centrocytoma, centroblastoma and in lymphoblastic leukaemia or lymphoma.

Down syndrome - transferrin parallels plasma iron changes.

The transferrin level was studied in patients with simple trisomy 21 and with Robertsonian unbalanced translocations 21/22 and 21/14. In all these groups of patients, known to have significantly lowered plasma iron levels, the transferrin levels were found to be decreased with respect to the control group.