Evolution of peptides that modulate the spectral qualities of bound, small-molecule fluorophores.

Background: Fluorophore dyes are used extensively in biomedical research to sensitively assay cellular constituents and physiology. We have created, as proof of principle, fluorophore dye binding peptides that could have applications in fluorescent dye-based approaches in vitro and in vivo.

Results: A panel of Texas red, Rhodamine red, Oregon green 514 and fluorescein binding peptides, termed here 'fluorettes', was selected via biopanning of a combinatorial library of 12-mer peptides fused to a minor coat pIII protein of the filamentous bacteriophage M13.

Interference of reovirus strains occurs between the stages of uncoating and dsRNA accumulation.

Interference of wild-type reovirus growth by some temperature-sensitive (ts) mutant viruses under non-permissive conditions or by other wild-type isolates has been demonstrated; however, the stage of the virus replication cycle at which interference occurs has not been defined. Examination of the time-course of the yields of T1 Lang (T1L) dsRNA in the progeny of mixed infections of T1L with T3 Dearing (T3D) or with a panel of T3D ts mutants at a non-permissive temperature revealed that interference takes place by 8-10 h post-infection and occurs prior to or at the same time as accumulation of reovirus dsRNA. Taken together with our previous results, these data indicate that interference occurs during a window between virus uncoating and synthesis of dsRNA in the reovirus replication cycle, probably at the stage of assembly of primary reovirus particles.

Evidence for phenotypic mixing with reovirus in cell culture.

From pairwise mixed infections of different reovirus wild-type isolates (T3 Dearing plus T1 Lang or plus T2 Jones) the progeny virus is phenotypically mixed, i.e., progeny viral particles contain proteins derived from both parents but the corresponding genes derived from only one parent.

Interference following mixed infection of reovirus isolates is linked to the M2 gene.

Following infection by pairs of reovirus isolates consisting of combinations of reovirus T1 Lang, T2 Jones, or T3 Dearing, we found that one of the isolates interfered with the yield of progeny RNA derived from the other parents. The most significant interference was produced by T2 Jones or T3 Dearing, when mixed with T1 Lang. Genetic analysis revealed that the presence of the M2 gene in the interfering parent (in the T1 Lang x T3 Dearing pair) was linked to interference.

All species of the genus Bordetella contain genes for pertussis toxin of Bordetella pertussis.

A molecular probe for the PT-operon of B. pertussis hybridized with 4.7 Kb EcoRI-fragments of chromosomal DNAs of B.

Bacteriophages of Bordetella sp.: features of lysogeny and conversion.

It has been the purpose of this paper to study molecular-biological features of the Bordetella bacteriophage interaction with the host cell during lysogeny and conversion as well as to determine the degree of homology between genomes of homologous and heterologous bacteriophages. Genomes of bacteriophages from B. pertussis 134, 41405 and B.

[Detection of the gene sequences of the leukocytosis (lymphocytosis)-stimulating factor of Bordetella pertussis in Bordetella parapertussis and Bordetella bronchiseptica by using molecular hybridization].

The 4.7 Kb EcoRI-fragment of phase I B. pertussis 475 (serovar 1.

[Cloning and gene expression of the leukocytosis (lymphocytosis) stimulating factor of Bordatella pertussis in Escherichia coli by bringing the PT genes close to the lactose promoter of plasmid pUC19].

The 4.7 Kb EcoRI-fragment of phase I B. pertussis 475 (serovar 1.

[Identification of protein products of the operon for leukocytosis (lymphocytosis)-stimulating factor from Bordetella pertussis cloned in Escherichia coli].

The hybrid plasmid pRH119 was constructed on the basis of vector plasmid pUC19 and shown to carry Bordetella pertussis PT operon in the same transcriptional orientation with the lac-promoter of the vector plasmid. Expression of PT genes in E. coli cells harbouring pRH119 was not registered.

[Lysogeny and conversion characteristics of microbes in the genus Bordetella].

The genomes of B. pertussis bacteriophages 134 and 41405 and B. bronchiseptica bacteriophage 214 have been studied.

[Cloning of the genes of hemolytic factors of Bordetella pertussis in Escherichia coli].

The gene library of B. pertussis strain No. 475 (serovar 1.

[Cloning of the hemagglutinin gene of influenza virus of subtype H3 in E. coli].

dsDNA of the influenza virus subtype A/Leningrad/385/80/R (H3N2)-recombinant A/Leningrad/385/80 (H3N2) and RR/8/34 (H1N1) has been synthesized using polyadenylated viral RNA as a template. This dsDNA has been cloned on plasmid pUC19. A clone has been selected harbouring the plasmid with included proximal fragment of hemagglutinin gene that contains the main antigenic determinants.

[Properties of T-cell growth factor obtained from diucifon-stimulated human lymphocytes].

The properties of T-cell growth factor (TCGF), obtained by diucifon (Dc) stimulation of human mononuclear cells (MNC) (TCGF-Dc) have been studied. Taking into account the fact that Dc alone does not, like other TCGF inductors, cause proliferation, differences between TCGF-Dc and TCGF were suggested. Partial purification of supernatant from cells, activated by Dc was performed on Sephadex G-100 column.

[Cloning and the expression of the hemolysin gene of Leptospira pomona pomona in Escherichia coli].

The library of Leptospira pomona genes was obtained on phage vector AL 47.1. From this library a recombinant phage carrying the hemolysin gene was selected.

[Molecular cloning of the structural genes of the Escherichia coli deo-operon in plasmid RSF2124].

A specialized phage lambda ddeo carrying the deo operon of Escherichia coli is analyzed by exposing the DNA to the specific restriction endonucleases EcoRI and BamHI. Using the lambda ddeo DNA fragment, obtained by digestion with BamHI and plasmid RSF2124 as a vehicle, the hybrid plasmid pAM1 carrying all the genes of the deo operon is constructed and cloned in E. coli cells.