This study aimed to examine the effect of obstructive cholestasis on the pharmacokinetics of digoxin. Eighteen male rabbits were randomly ascribed to the two study groups: the sham-operated control group and the examined group - with common and cystic bile duct ligations. Digoxin was administered intravenously as a single dose of 0.
View Article and Find Full Text PDFA change in the functioning of the liver as a result of experimental cholestasis could result in a change in the biotransformation of drugs. The aim of this study was to evaluate the effect of extrahepatic cholestasis on the pharmacokinetics of digoxin. The investigation was performed on male rabbits randomly divided into two groups: sham-operated and animals with bile-duct ligation.
View Article and Find Full Text PDFThirty male mongrel rabbits were divided into 3 equal groups: (1) controls, (2) animals receiving a high-fat diet (HFD) containing cholesterol and coconut oil, and (3) HFD + lecithin. The experiment lasted 12 weeks. In animals receiving a HFD (group II) the QRS interval (ECG) was significantly prolonged, and the concentration of lipid fractions (except for HDL-cholesterol) and malondialdehyde (MDA) in the blood was markedly elevated.
View Article and Find Full Text PDF1. An altered functional state of liver due to experimental cholestasis could result in a change in the biotransformation of drugs. The aim of this study was to evaluate an influence of obstructive cholestasis on the pharmacokinetics of phenazone (antipyrine).
View Article and Find Full Text PDFA group of 30 male mongrel rabbits was divided into 3 subgroups: controls, animals receiving a high-fat diet (HFD) containing cholesterol and coconut oil, HFD + Extr. Fagopyri (EF) were treated for 12 weeks. Surface areas of lipid deposites after 12 weeks of treatment measured planimetrically in the intima of the aorta, averaged 86.
View Article and Find Full Text PDFThe aim of this study was to evaluate, using an experimental model, the effect of obstructive cholestasis on the pharmacokinetics of lidocaine and the formation rate of its major metabolite- monoethylglycinexylidide (MEGX)-in rabbits. The investigation was carried out on 20 rabbits, randomly divided into two groups: a control one sham-operated and an experimental one-animals with biliary duct ligation. The measurements, i.
View Article and Find Full Text PDFFolia Histochem Cytobiol
June 1996
Electron microscopic studies were performed to investigate the influence of chronic lead acetate treatment on morphology of rat epididymis. Dense, lead-loaded inclusions were found in the cytoplasm of epididymal principal cells, especially in the caput of epididymis. They were also present, but in smaller amounts, in smooth muscle cells.
View Article and Find Full Text PDFForty male mongrel rabbits were divided into 4 equal groups: (1) controls, (2) animals receiving a high-fat diet (HFD) containing cholesterol and coconut oil, (3) HFD + standardized. Ginkgo biloba extract (GB), and (4) HFD + rutin (as a well known substance it was used for comparison of pure single flavonol diglycoside activity with total extract). The experiment lasted 12 weeks.
View Article and Find Full Text PDFMorphology of sperm phagocytosing rat peritoneal macrophages (PM) in control and Lipiodol added cultures was evaluated under light as well as scanning electron and transmission electron microscopes. The spermiophagic index (SPI) was assessed to estimate an inhibitory effect of Lipiodol on sperm phagocytosis to be compared with the outcome of morphological studies. Lipiodol inhibits in vitro sperm phagocytosis as indicated by SPI and evaluation under light, scanning electron and transmission electron microscopes.
View Article and Find Full Text PDFThe aim of this study was to investigate the effect of unilateral nephrectomy on the pharmacokinetics of gentamicin. The study was carried out on 19 male rabbits. Gentamicin was injected i.
View Article and Find Full Text PDFFolia Histochem Cytobiol
October 1993
The relation was studied between the morphology of tests and epididymides on the one hand, and the lead content in these organs on the other. The testes of rats, which for the time of 5 spermatogeneses (9 months) were drinking 1% lead acetate(II), displayed all generations and layers of spermatogenic cells at respective stages of the seminiferous epithelium cycle. The lead content in testes of the animals did not differ significantly from the value of this element in gonads of control rats.
View Article and Find Full Text PDFParacetamol causes extensive liver damage when taken in overdose quantities; however, it is less hepatotoxic when administered in combination with caffeine. The present work in mice was undertaken to study the effect of caffeine on mortality rates and biochemical and histological parameters of liver damage after administration of toxic doses of paracetamol. It was found that caffeine markedly increased the survival rate after administration of a dose of paracetamol that was lethal to 50% and 100% of mice, reduced liver damage as assessed by serum glutamic pyruvic and glutamic oxaloacetic transaminase activities, partially prevented the depletion of reduced glutathione and reduced histological changes to the liver accompanying paracetamol intoxication.
View Article and Find Full Text PDFMetoclopramide (MCP) in doses of 2.2 mg kg-1 body weight, being administered intraperitoneally to rats for 14 d, provokes a premature release of germ cells in the testes. The number of spermatozoa is reduced.
View Article and Find Full Text PDFAtherosclerosis was induced in male mongrel rabbits with a high-fat diet and the influence of essential phospholipids (EPL) on plaque formation, parameters of lipid metabolism and immunological functions was studied. When EPL were added to the high-fat diet there was a significant reduction in the area of atherosclerotic involvement of the aorta. The serum concentration of lipids decreased, often to normal values, and cholesterol esterified with polyunsaturated fatty acids appeared.
View Article and Find Full Text PDFThe effect of selenium and vitamin E on lipid level in the blood serum, on the oxygen free radicals generation and on morphology of heart was tested after separate and combined administration to mongrel male rabbits fed on a high-fat diet (HFD). The lipid level and oxygen free radical generation was depressed markedly in animals fed on a HFD and receiving simultaneously selenium and vitamin E. In animals on a HFD the walls of the heart vessels were thickened, always to their complete obliteration.
View Article and Find Full Text PDFMale mongrel rabbits, divided into 5 groups (1) controls, (2) animals receiving a high-fat diet (HFD) containing cholesterol and coconut oil, (3) HFD + selenium, (4) HFD + vitamin E, (5) HFD + selenium + vitamin E, were treated for 12 weeks. In the groups receiving selenium and/or vitamin E, the elevation of serum total lipids, beta-lipoproteins, total cholesterol and triglyceride was markedly suppressed. HDL cholesterol in these groups of animals was increased.
View Article and Find Full Text PDFThe high-fat diet consisted of cholesterol, hydrogenated coconut oil, and cholic acid. In the blood serum and in liver homogenate lipid content, cholesterol, and triglycerides were assayed. Cytochrome P-450 concentration in liver microsomes was also estimated.
View Article and Find Full Text PDFEndocrinol Exp
September 1989
Epithelial cells of the rat's epididymal caput were cultivated according to own modification of the Kierszenbaum's method [1981]. The said modification consisted in developing primary cultures of the epithelial cells in the epididymal duct by making use of small tubular segments instead of deisolated cells of the whole epididymal duct wall. Such small segments of the tubules were procured by resorting to mechanical isolation and a 4-grade enzymatic isolation with trypsin and collagenase, whereupon the produced suspension of cells and tubules was filtered through a grid, the meshes of which being 40 X 50 microns in diameter.
View Article and Find Full Text PDFThe performed evaluation covers the elastic fibre elements in the dermis of rabbits after the preparation of 30% elastin in propylene glycol was rubber in. Moreover, the effect of elastin on the degree of proliferation of fibroblasts, isolated from the rabbit's skin, was determined in vitro. It has been found out that the used preparation exerts a stimulating effect on elastogenesis, but no differences in the degree of proliferation of the fibroblasts under in vitro condition were observed.
View Article and Find Full Text PDFMale rats were subjected to 9-month-long exposure to ammonium fluoride. The performed evaluation covered the seminiferous epithelium and epididymis. The greatest changes in animals used in the experiment were observed in epididymis.
View Article and Find Full Text PDFThe aim of the performed studies has been to find out wether or not the ultrastructural alterations of the epithelial cells in the rat's epididymal caput occur in hyperprolactinemia induced by metoclopramide and to see if the observed changes are of reversible character. It has been revealed that prolactin concentration was twice as high as in control animals due to peritoneal administration of metoclopramide in a dose of 2.2 mg/kg body mass, given for 14 days.
View Article and Find Full Text PDFZinc content was determined separately in spermatozoa taken from epididymal caput and cauda in rats. It was revealed that spermatozoa transported from the epididymal caput to the cauda reduce about 54% of zinc. This reduction is significantly inhibited in spermatozoa of rats receiving metoclopramide.
View Article and Find Full Text PDFFolia Histochem Cytobiol
February 1986
A method has been developed for isolation of cells from the efferent ductules of the rat epididymis to obtain a population of epithelial cells. The technique depends on a transfer of segments of the ductules into a new isolation medium and gradual purification from fat cells, fibroblasts, collagen fibres and smooth muscle cells. Segments of the ductules so isolated were further separated into single cells or their aggregates.
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