Publications by authors named "Rozanne Sandri-Goldin"
Article Synopsis
- Viruses pose significant health challenges, leading to issues like respiratory infections, cancer, and neurological impairments, but virology research has developed vaccines and antivirals to mitigate these problems.
- The COVID-19 pandemic has heightened public scrutiny of virology, especially regarding the safe conduct of research with human pathogens, leading to confusion and misinterpretation about the origins of SARS-CoV-2.
- This article aims to clarify misconceptions by explaining gain-of-function research, the origins of SARS-CoV-2, and the regulatory frameworks in place, fostering informed discussions and emphasizing the need for balanced, evidence-based dialogue in virology.
Article Synopsis
- Viruses have historically caused serious health issues, including respiratory infections and cancer, leading to significant virology research that resulted in vaccines and antiviral treatments.
- The COVID-19 pandemic highlighted the necessity for careful research on human pathogens, creating both concerns and confusion about the safety of virology work and the origins of SARS-CoV-2.
- The article aims to clarify misunderstandings by explaining gain-of-function research, exploring the origins of SARS-CoV-2, and discussing regulatory oversight, while advocating for rational and evidence-based discussions to guide policy decisions in virology.
Article Synopsis
- Viruses pose significant health challenges, leading to various issues such as respiratory infections and cancer, prompting virology research to develop vaccines and antiviral treatments over the past 60+ years.
- The COVID-19 pandemic has intensified focus on virology, bringing up safety concerns about research involving human pathogens and creating public confusion between safe research practices and the origins of SARS-CoV-2.
- The article aims to clarify these issues by discussing gain-of-function research, the origins of SARS-CoV-2, and current regulatory frameworks, advocating for informed, balanced conversations to support necessary virology research.
Eukaryotic gene expression is extensively regulated by cellular stress and pathogen infections. We have previously shown that herpes simplex virus 1 (HSV-1) and several cellular stresses cause widespread disruption of transcription termination (DoTT) of RNA polymerase II (RNAPII) in host genes and that the viral immediate early factor ICP27 plays an important role in HSV-1-induced DoTT. Here, we show that HSV-1 infection also leads to widespread changes in alternative polyadenylation (APA) of host mRNAs.
View Article and Find Full Text PDFViral tracers are important tools for neuroanatomical mapping and genetic payload delivery. Genetically modified viruses allow for cell-type-specific targeting and overcome many limitations of non-viral tracers. Here, we summarize the viruses that have been developed for neural circuit mapping, and we provide a primer on currently applied anterograde and retrograde viral tracers with practical guidance on experimental uses.
View Article and Find Full Text PDFInfection by viruses, including herpes simplex virus-1 (HSV-1), and cellular stresses cause widespread disruption of transcription termination (DoTT) of RNA polymerase II (RNAPII) in host genes. However, the underlying mechanisms remain unclear. Here, we demonstrate that the HSV-1 immediate early protein ICP27 induces DoTT by directly binding to the essential mRNA 3' processing factor CPSF.
View Article and Find Full Text PDFSerine-arginine (SR) protein kinase 1 (SRPK1) catalyzes the phosphorylation of SR proteins, which are a conserved family of splicing factors that contain a domain rich in arginine and serine repeats. SR proteins play important roles in constitutive pre-mRNA splicing and are also important regulators of alternative splicing. During herpes simplex virus infection, SRPK1 is inactivated and its cellular distribution is markedly altered by interaction with the viral protein ICP27, resulting in hypophosphorylation of SR proteins.
View Article and Find Full Text PDFKaposi's sarcoma-associated herpesvirus (KSHV) transcribes a long noncoding polyadenylated nuclear (PAN) RNA, which promotes the latent to lytic transition by repressing host genes involved in antiviral responses as well as viral proteins that support the latent state. KSHV also expresses several early proteins including ORF57 (Mta), a member of the conserved multifunctional ICP27 protein family, which is essential for productive replication. ORF57/Mta interacts with PAN RNA via a region termed the Mta responsive element (MRE), stabilizing the transcript and supporting nuclear accumulation.
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