Effect of Seasonal Decrease in Temperature on the Content and Composition of Guayulins in Stems of Guayule (, Gray).

The guayulins are a family of sesquiterpene compounds that consist of an isoprenoid nucleus substituted either by -cinnamic or -anisic acid, and are present only in the resinous fraction of the rubber plant guayule (, Gray). While the natural role of the guayulins remains enigmatic, they may serve as a defense function against other plants or herbivores by virtue of the accumulation of cinnamic acid. Prior research has suggested seasonal variation in guayulin content, which has been shown to decrease as winter arrives in two different varieties.

Adapting the Accelerated Solvent Extraction Method for Resin and Rubber Determination in Guayule Using the BÜCHI Speed Extractor.

Guayule ( Gray) is a promising alternative source to for the production of natural rubber, which can reach levels of 8-9% under industrialized farming conditions. The most common method for determining rubber concentration is by accelerated solvent extraction (ASE), a technique developed by the Dionex Corporation and almost exclusively performed with the Dionex ASE-200 or 350 systems. Herein, it is sought to apply and adapt the most common methods used in the literature for the Dionex system to another extraction platform, the BÜCHI Speed Extractor E-914.