Publications by authors named "Rozainanee M Zain"
Article Synopsis
- - CHIKV cases in Malaysia increased from 2019 to 2021, likely due to the virus spreading from neighboring countries and a genotype shift from earlier outbreaks, particularly linked to the Indian Ocean Islands outbreak in 2004.
- - An analysis of 56 CHIKV positive serum samples from Tangkak, Johor highlighted that the 2021 outbreak strains bore an ECSA-IOL genotype, closely resembling strains from a 2018-2019 outbreak in Thailand and showing mutation adaptations favoring different mosquito vectors.
- - The findings suggest the 2021 outbreak in Malaysia is connected to strains from the Indian subcontinent via Thailand, with notable genetic mutations potentially enhancing the virus's ability to spread and adapt to
The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral anti-gens varies within populations following natural infection. Here, we designed a quantitative, multi-epitope protein microarray comprising various nucleocapsid protein structural motifs, including two structural domains and three intrinsically disordered regions.
View Article and Find Full Text PDFObjective: To determine drug resistance mutations and the HIV-1 subtypes among antiretroviral treatment naive HIV-1 patients in Peninsular Malaysia.
Methods: A total of 45 samples from four hospitals that provide HIV viral load services were subjected to the amplification of the protease and two third of reverse transcriptase regions of the pol gene by RT-PCR and Sanger sequencing. Drug resistance mutation (DRM) interpretation reports the presence of mutations related to protease inhibitors (PIs), Nucleoside reverse-transcriptase inhibitors (NRTI) and Non-nucleoside reverse-transcriptase inhibitors (NNRTI) based on analysis using Stanford HIV database program.
Sharing experiences from a reference laboratory in the public health response for Ebola viral disease, MERS-CoV and H7N9 influenza virus investigations.
Asian Pac J Trop Med
February 2016
An efficient public health preparedness and response plan for infectious disease management is important in recent times when emerging and exotic diseases that hitherto were not common have surfaced in countries with potential to spread outside borders. Stewardship from a reference laboratory is important to take the lead for the laboratory network, to proactively set up disease surveillance, provide referral diagnostic services, on-going training and mentorship and to ensure coordination of an effective laboratory response. In Malaysia, the Institute for Medical Research has provided the stewardship for the Ministry of Health's laboratory network that comprises of hospital pathology, public health and university laboratories.
View Article and Find Full Text PDFBackground: The Elecsys® HIV combi PT assay was developed to allow earlier detection of HIV infection with increased sensitivity and specificity.
Objectives: To validate the assay for screening and reliable early detection of HIV-1 infection in Asia.
Study Design: Samples tested reflected those routinely screened in Asia and comprised: HIV-1 antigen lysate (25 samples) and antibody (20 samples) dilutions; seven HIV-1 seroconversion panels (46 samples); 39 patient samples from early infection; 183 known-positive sera; HIV-1 p24 antigen sensitivity panel (seven samples); >500 routine clinical samples per center.