Voltammetric immunosensor for E-cadherin promoter DNA methylation using a FeO-citric acid nanocomposite and a screen-printed carbon electrode modified with poly(vinyl alcohol) and reduced graphene oxide.

Silencing of tumor suppressor genes (E-cadherin) by promoter DNA methylation may lead to the development of invasive phenotypes in epithelial tissues. The authors describe an electrochemical nanobiosensor for early detection and screening of circulating methylated DNA as a biomarker for cancers. First, the antibody against 5-methylcytosine was physically immobilized onto modified with reduced graphene oxide and polyvinylalcohol.