Pharmacokinetics of small hyperbranched polyglycerols as an osmotic agent for peritoneal dialysis: Plasma exposure, organ distribution and excretion in rats.

Background: Small hyperbranched polyglycerol (HPG) has been recently of interest for peritoneal dialysis, but its pharmacokinetics is barely understood. This study investigated the absorption, distribution and excretion of 1 and 3 kDa HPG.

Methods: Rats (naive, 5/6 nephrectomy (5/6 Nx) or bilateral nephrectomy (BNx)) received a single dose of H-labelled HPG-containing solutions intraperitoneally (IP) or intravenously (IV).

Simple capillary electrophoresis-mass spectrometry method for complex glycan analysis using a flow-through microvial interface.

A flow-through microvial is used to interface capillary electrophoresis and mass spectrometry (CE-MS) to develop a method for simultaneous profiling both neutral and sialylated glycans without derivatization or labeling. The CE separation was performed at near-zero electroosmotic flow in a capillary with neutral, hydrophilic coating, using 50 mM ammonium acetate in 20% methanol (pH 3.1) as the background electrolyte.

Capillary electrophoresis-mass spectrometry using a flow-through microvial interface for cationic metabolome analysis.

The application of CE-MS in the field of metabolomics is underrepresented, even though it is in principle highly suited for the analysis of small charged compounds, as many metabolites are. Moreover, a robust coupling, using the sheath liquid (SL)-assisted interface was already presented more than a decade ago. A lack of concentration sensitivity is often mentioned as a reason for the underrepresentation of CE-MS in metabolomics.

Micro- and macroheterogeneity of N-glycosylation yields size and charge isoforms of human sex hormone binding globulin circulating in serum.

Human sex hormone binding globulin (hSHBG) is a serum glycoprotein central to the transport and targeted delivery of sex hormones to steroid-sensitive tissues. Several molecular mechanisms of action of hSHBG, including the function of its attached glycans remain unknown. Here, we perform a detailed site-specific characterization of the N- and O-linked glycosylation of serum-derived hSHBG.

Capillary electrophoresis mass spectrometry for the characterization of O-acetylated N-glycans from fish serum.

O-acetylated N-glycans from fish serum of Atlantic salmon (Salmo salar) are characterized by capillary electrophoresis (CE) in conjunction with both laser-induced fluorescence (LIF) and mass spectrometry (MS) detection methods. Glycans derivatized with negatively charged fluorescent label 8-aminopyrene-1,2,6-trisulfonate (APTS) were separated to obtain a CE-LIF profile of the complex glycan mixture, and the profile concurs with that obtained by using electrospray mass spectrometry. The identity of the APTS-labeled glycans was confirmed by CE-MS.

A promising capillary electrophoresis-electrospray ionization-mass spectrometry method for carbohydrate analysis.

A method for adapting widely used CE conditions for the separation of fluorescently labeled carbohydrates to permit online ESI-MS detection is presented. Reverse polarity separations were performed in bare fused-silica capillaries with an acidic BGE. Under these conditions, negatively charged 8-aminopyrene 1,3,6-trisulfonate-labeled carbohydrates migrate forward against the EOF, which is towards the capillary inlet.