Expression of leptin mRNA as non-invasive biomarker in type 2 diabetes mellitus.

Context: Leptin, an adipocyte-derived signalling molecule that plays important role in the regulation of energy balance, body weight and glucose metabolism.

Objective: To evaluate leptin mRNA expression as a predictive biomarker in type 2 diabetes mellitus (T2DM).

Materials And Methods: To detect the expression patterns of leptin mRNA, a quantitative real-time polymerase chain reaction was performed on samples collected from 71 T2DM patients and 32 non-diabetic controls.

Intergenic lnc-LEP-2:6 and lnc-LEP-2:7 as novel biomarkers associated with type 2 diabetes mellitus.

Context: Type 2 diabetes mellitus (T2DM) has undeniably become a significant threat to public health in the modern world.

Objective: To identify molecules involved in the development of T2DM, the expression patterns of cis-lincRNAs-LEP were investigated in T2DM patients.

Materials And Methods: The expression levels of 11 lncRNAs-LEP and mRNA in 71 diabetic patients and 32 controls were determined using qRT-PCR.

Ghrelin intronic lncRNAs, and , as novel biomarkers in type 2 diabetes mellitus.

Background: We aim to identify circulating lncRNAs located in the region of the () gene that play a role in the development of T2DM.

Methods: Bioinformatic tool was used to identify candidates -lncRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to compare the expression levels of selected lncRNAs on diabetic patients and non-diabetic controls.

Polymorphisms in RETN gene and susceptibility to colon cancer in Saudi patients.

Background And Objectives: Resistin is an adipocytokine, which has been studied for its role in insulin resistance and recently in inflammation. Several single-nucleotide polymorphisms (SNPs) have been identified in the human resistin gene (RETN). This study aims to investigate the association of RETN rs1862513 (C-420G) and rs3745367 (G+299A) SNPs with the colon cancer risk in Saudi patients.

The adiponectin gene, ADIPOQ, and genetic susceptibility to colon cancer.

In order to evaluate the contribution of polymorphisms of the adiponectin gene, ADIPOQ, to the risk of colon cancer, we conducted a case-control study of 60 colon cancer patients and 60 age, gender and ethnicity-matched controls in the Saudi population. We tested the hypothesis by analyzing the genotypes for two single nucleotide polymorphisms (SNPs), rs1501299 (G276T) and rs2241766 (T45G), in the ADIPOQ gene. In addition, the study was also designed to assess whether the two SNPs contribute to circulating adiponectin levels.

Resistin in human colon cancer. Increased expression independently of resistin promoter C-180G genotype.

Objective: To determine the relationship between resistin gene RETN C-180G variant and circulating resistin concentration in Saudi colon cancer patients.

Methods: This case-control study was conducted in the Biochemistry Department, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia from April 2009 to December 2009. The serum concentration had been measured with enzyme-linked immunosorbent assay in 60 colon cancer patients and in 60 controls matched in gender and age.

Correlation of leptin and sex hormones with endocrine changes in healthy Saudi women of different body weights.

Background: A relationship between estrogen and leptin has been described during the follicular phase of both spontaneous menstrual cycles and cycles stimulated with exogenous follicle-stimulating hormone (FSH), which suggest that leptin has either a direct effect on or is regulated by gonadal steroids in the human ovary. To examine the changes in plasma leptin levels during the menstrual cycle, we studied the association between plasma leptin and reproductive hormones in young, healthy Saudi women.

Subjects And Methods: Sixty-five young women between 19 to 39 years of age, with a normal menstrual cycle, were grouped into 33 overweight and obese females of BMI >25 kg/m2, and 32 lean females of BMI <25 kg/m2.

Serum resistin, adiposity and insulin resistance in Saudi women with type 2 diabetes mellitus.

Background: The role of adipocyte hormones in modulating insulin sensitivity and glucose tolerance are of increasing interest and importance in studies of type 2 diabetes mellitus. Recently a unique signaling molecule, resistin, has been proposed as playing a role in the pathogenesis of obesity-related insulin resistance, but its relevance to human diabetes remains uncertain. Therefore, we assessed the relationship between serum resistin concentrations and insulin resistance in lean, overweight and obese (OW/OB) non-diabetic and diabetic Saudi women.

Relationship of leptin concentration to gender, body mass index and age in Saudi adults.

Objective: Leptin concentrations are highly correlated with body fat storage and exhibit sexual dimorphism, with women having higher concentrations at every level of relative or absolute adiposity. To test whether or not this relation is consistent across the Saudi population. This study aims to investigate the effect of gender, obesity related parameters, and age on leptin levels from representative samples of Saudi women and men.