Evaluation of Microleakage in Resin Composites Bonded to an Er:YAG Laser and Bur-Prepared Root and Coronal Dentin Using Different Bonding Agents.

This study was conducted to assess the microleakage in Er:YAG laser-ablated and bur-prepared root and coronal dentin cavities using self-etch and total-etch adhesive systems. Sixty extracted caries-free human third molars were sectioned for dentin exposure. Then, two standard class V cavities were prepared in the root and coronal dentin of each tooth and allocated to one of the following conditioning groups randomly (n=12/Group): G1: Diamond bur for cavity preparation and single bond (BESB) etch-and-rinse adhesive for bonding, G2: Er:YAG laser (160 mJ, 20 Hz, 29.

The role of CRISPR-Cas in advancing precision periodontics.

The significant advancement of molecular biology has revolutionized medicine and provided important technologies to further clinical research development. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are DNA sequences derived from bacteriophages which have previously infected the bacterial species. The CRISPR-Cas system plays a key role in bacterial defense by detecting and destroying DNA fragments during subsequent bacteriophage invasions.

Nitrogen-Based Lewis Acids Derived from Phosphonium Diazo Cations.

Reaction of PPh and [(p-ClC H )N ][BF ] affords [(p-ClC H )N(PPh )N(PPh )][BF ] 1, while reaction with (Ph PCH ) gave [(p-ClC H )(NPh PCH ) )][BF ] 2. These species confirm the Lewis acidity of [(p-ClC H )N (PR )][BF ] cations at N. In contrast, use of bulky phosphines afford the species [ArN (PR )][BF ] (R=tBu 3, Mes 4).