Phylogenetics of Lepidonotopodini (Macellicephalinae, Polynoidae, Annelida) and Comparative Mitogenomics of Shallow-Water vs. Deep-Sea Scaleworms (Aphroditiformia).

Within Polynoidae, a diverse aphroditiform family, the subfamily Macellicephalinae comprises anchialine cave-dwelling and deep-sea scaleworms. In this study, Lepidonotopodinae is synonymized with Macellicephalinae, and the tribe Lepidonotopodini is applied to a well-supported clade inhabiting deep-sea chemosynthetic-based ecosystems. Newly sequenced "genome skimming" data for 30 deep-sea polynoids and the comparatively shallow living is used to bioinformatically assemble their mitogenomes.

Description of a new (Annelida, Polychaeta, Siboglinidae) species colonizing cow bones in the South Atlantic Ocean.

A new species of is described here using molecular and morphological data. It was found at the depth of 550 m off the Brazilian coast through experimental deployment of cow bones. is the second species from the Southwest Atlantic Ocean and had been previously reported as 'BioSuOr-4'.

The mitogenomes of two species of sea pigs, and (Elasipodida: Holothuroidea: Echinodermata).

Mitochondrial genomes of and are presented, each with 13 protein coding genes, two rRNA genes, and 22 tRNA genes. That of has 15,909 base pairs and that of is 15,896 base pairs. There is a suspected tandem repeat region of undetermined length in the assemblies of both and .

Three New Species of Deep-Sea Wood-Associated Sea Stars (Asteroidea: Caymanostellidae) from the Eastern Pacific.

Caymanostellidae is a group of rarely collected and morphologically unusual sea stars that have been exclusively encountered on wood falls in the deep sea. There are currently three genera and seven species described, occurring in the Atlantic, Pacific and Indian Oceans with a depth range between 418 and 6780 m. Three new species are here described from specimens collected from wood falls in multiple localities across the Pacific margin of Costa Rica and near the Gulf of California (Mexico): Caymanostella scrippscognaticausa sp.

New Species of Osedax (Siboglinidae: Annelida) from New Zealand and the Gulf of Mexico.

Osedax is now known to be distributed around the world with more than 30 named and undescribed species. Here we report the discovery of four new species from two localities: Osedax bozoi n. sp.

Taxonomy and phylogeny of the family Suberitidae (Porifera: Demospongiae) in California.

This study presents a comprehensive taxonomic revision of the family Suberitidae (Porifera: Demospongiae) for California, USA. We include the three species previously known from the region, document two additional species previously known from other regions, and formally describe four new species as Pseudosuberites latke sp. nov.

Deep-ocean macrofaunal assemblages on ferromanganese and phosphorite-rich substrates in the Southern California Borderland.

Mineral-rich hardgrounds, such as ferromanganese (FeMn) crusts and phosphorites, occur on seamounts and continental margins, gaining attention for their resource potential due to their enrichment in valuable metals in some regions. This study focuses on the Southern California Borderland (SCB), an area characterized by uneven and heterogeneous topography featuring FeMn crusts, phosphorites, basalt, and sedimentary rocks that occur at varying depths and are exposed to a range of oxygen concentrations. Due to its heterogeneity, this region serves as an optimal setting for investigating the relationship between mineral-rich hardgrounds and benthic fauna.

Safety of Peripherally Infused Sympathomimetic Vasopressors in the Intensive Care Unit and Emergency Department.

Background: Sympathomimetic vasopressors may be administered through a peripheral catheter, but there are limited data available on the safety of peripheral use.

Objective: The purpose of this study was to analyze the safety of peripherally infused sympathomimetic vasopressors.

Methods: A multicenter, retrospective observational study was conducted to evaluate patients who received peripheral vasopressors.

Comparison of Tenecteplase Versus Alteplase for the Treatment of Pulmonary Embolism and Cardiac Arrest with Suspected Pulmonary Embolism.

High-risk pulmonary embolism (PE) is a life-threatening disease state with current guidelines recommending reperfusion therapy with systemic thrombolytics in addition to anticoagulation. This was a prospective observational cohort study with a historical control group comparing tenecteplase to alteplase for the treatment of PE or cardiac arrest with suspected PE. The primary outcome was the incidence of institutional protocol deviations defined as incorrect thrombolytic dose administered or the incorrect product compounded.

Waste barrel contamination and macrobenthic communities in the San Pedro Basin DDT dumpsite.

Industrial waste barrels were discarded from 1947 to 1961 at a DDT dumpsite in the San Pedro Basin (SPB) in southern California, USA at ~890 m. The barrels were studied for effects on sediment concentrations of DDX, PCBs, PAHs and sediment properties, and on benthic macrofaunal assemblages, including metazoan meiofaunal taxa >0.3 mm.

The mitochondrial genome of the deep-sea pyramid urchin (Echinoidea: Holasteroida: Pourtalesiidae).

We present the mitochondrial genome of the deep-sea, epibenthic, irregular echinoid , representing the first sequenced mitogenome of the order Holasteroida. The length of the complete mitochondrial genome is 15,716 base pairs, and its GC content is 34.87%.

A remarkable new deep-sea nereidid (Annelida: Nereididae) with gills.

Nereidid polychaetes are well known from shallow marine habitats, but their diversity in the deep sea is poorly known. Here we describe an unusual new nereidid species found at methane seeps off the Pacific coast of Costa Rica. Specimens of Pectinereis strickrotti gen.

Range extensions of Pacific bone-eating worms (Annelida, Siboglinidae, Osedax).

First described in 2004 off California, worms are now known from many of the world's oceans, ranging from 10 to over 4000 m in depth. Currently, little is known about species ranges, since most descriptions are from single localities. In this study, we used new sampling in the north-eastern Pacific and available GenBank data from off Japan and Brazil to report expanded ranges for five species: , , , and .

Two new species of (Annelida: Hesionidae) associated with ghost shrimps (Crustacea: Decapoda) and their phylogenetic relationships.

Two new species of Hesionidae, sp. nov. and sp.

Phylogeny and taxonomy of Himerometroidea (Echinodermata: Crinoidea).

Himerometroidea is a clade of chiefly shallow-water, tropical, feather-star crinoids that is currently divided, based on morphology, into four families comprising 119 extant species in 31 genera. Our molecular phylogenetic results, based on three mitochondrial (CO1, 16S, CytB) and two nuclear (ITS and 28S) markers for 55 accepted species in 23 of the extant genera, allow for six clades within Himerometroidea to be given family ranks. Maximum parsimony, maximum likelihood, and Bayesian inference analyses recovered largely congruent topologies with varying nodal support.

Updated phylogeny of Vestimentifera (Siboglinidae, Polychaeta, Annelida) based on mitochondrial genomes, with a new species.

Siboglinid tubeworms are found at chemosynthetic environments worldwide and the Vestimentifera clade is particularly well known for their reliance on chemoautotrophic bacterial symbionts for nutrition. The mitochondrial genomes have been published for nine vestimentiferan species to date. This study provides new complete mitochondrial genomes for ten further Vestimentifera, including the first mitochondrial genomes sequenced for Alaysia spiralis, Arcovestia ivanovi, Lamellibrachia barhami, Lamellibrachia columna, Lamellibrachia donwalshi, and unnamed species of Alaysia and Oasisia.

Confusion will be my epitaph: genome-scale discordance stifles phylogenetic resolution of Holothuroidea.

Sea cucumbers (Holothuroidea) are a diverse clade of echinoderms found from intertidal waters to the bottom of the deepest oceanic trenches. Their reduced skeletons and limited number of phylogenetically informative traits have long obfuscated morphological classifications. Sanger-sequenced molecular datasets have also failed to constrain the position of major lineages.

A dynamic epibiont community associated with the bone-eating polychaete genus .

the deep-sea annelid found at sunken whalefalls, is known to host Oceanospirillales bacterial endosymbionts intracellularly in specialized roots, which help it feed exclusively on vertebrate bones. Past studies, however, have also made mention of external bacteria on their trunks. During a 14-yr study, we reveal a dynamic, yet persistent, shift of Campylobacterales integrated into the epidermis of , which change over time as the whale carcass degrades on the sea floor.

Range-wide population genomics of common seadragons shows secondary contact over a former barrier and insights on illegal capture.

Background: Common seadragons (Phyllopteryx taeniolatus, Syngnathidae) are an emblem of the diverse endemic fauna of Australia's southern rocky reefs, the newly recognized "Great Southern Reef." A lack of assessments spanning this global biodiversity hotspot in its entirety is currently hampering an understanding of the factors that have contributed to its diversity. The common seadragon has a wide range across Australia's entire temperate south and includes a geogenetic break over a former land bridge, which has called its status as a single species into question.

Distinct genomic routes underlie transitions to specialised symbiotic lifestyles in deep-sea annelid worms.

Bacterial symbioses allow annelids to colonise extreme ecological niches, such as hydrothermal vents and whale falls. Yet, the genetic principles sustaining these symbioses remain unclear. Here, we show that different genomic adaptations underpin the symbioses of phylogenetically related annelids with distinct nutritional strategies.

First known observations of brooding, development, and hatching of fertilized eggs for the North Pacific bigeye octopus, .

The North Pacific bigeye octopus, (Berry, 1911) is a cold-water, deep-sea octopod. Little is known about their biology due to difficulty accessing their natural habitat and obtaining live specimens. Although they are a frequent bycatch product in commercial bottom trawl fisheries, individuals of this species have rarely been raised in captivity and their embryonic development has not yet been documented.

Vampire Worms; A revision of Galapagomystides (Phyllodocidae, Annelida), with the description of three new species.

Galapagomystides is an exclusively deep-sea group of Phyllodocidae, originally erected for Galapagomystides aristata from hydrothermal vents of the Galapagos Rift. In this study, Phyllodocidae collected from hydrothermal vents and methane seeps from the Pacific Ocean, including specimens from vents of the East Pacific Rise identified as Galapagomystides were studied using morphology (light microscopy and scanning electron microscopy) and DNA sequence data. Phylogenetic analysis of the newly generated molecular data (cytochrome c oxidase subunit I, 16S rRNA, 18S rRNA, and 28S rRNA) combined with an already available extensive dataset for Phyllodocidae resulted in a monophyletic Galapagomystides comprising five species.

The mitochondrial genome of the deep-sea axiid shrimp, (Decapoda: Axiidae).

Here, we present the first mitochondrial genome of . The mitogenome contains 13 protein-coding genes (PCGs), two rRNA genes, and 22 tRNA genes. The total length of the complete mitochondrial genome is 16,212 base pairs, and the GC content is 26.

Does substrate matter in the deep sea? A comparison of bone, wood, and carbonate rock colonizers.

Continental margins host methane seeps, animal falls and wood falls, with chemosynthetic communities that may share or exchange species. The goal of this study was to examine the existence and nature of linkages among chemosynthesis-based ecosystems by deploying organic fall mimics (bone and wood) alongside defaunated carbonate rocks within high and lesser levels of seepage activity for 7.4 years.