Evaluation of cisplatin-DNA crosslinks formation with UV-C application by the alkaline Comet-assay.

Unlabelled: The AIM of the present study was to investigate DNA damage induced by widely applied anticancer preparation cisplatin (cis-DDP) at doses comparable with therapeutic ones in leukocytes of healthy donors.

Methods: DNA damage and repair was estimated by the single cell gel electrophoresis or Comet-assay. For estimation of cis-DDP-induced DNA crosslinks the combined treatment by cis-DDP with the DNA damaging agent UV-C was used.