Comparison of the host cells apoptosis induced by precocious strains and virulent strains of Eimeria tenella.

The present study aims to investigate the similarities and differences between the host cells apoptosis induced by virulent line of Eimeria tenella (Tsx) and precocious line (PTsx), which can provide a theoretical basis for the study of drugs and vaccines against coccidiosis. HE staining, Hoechst 33342/AnnexinV-FITC/PI composite staining, and ELISA were used to detect the infection rate, apoptosis rate, and Caspase-3 enzyme activity of host cells infected by PTsx or Tsx, respectively. The apoptotic rates and Caspase-3 absorbance of the inoculation groups were lower (P < 0.

Effect of ATP and Bax on the apoptosis of Eimeria tenella host cells.

Background: Eimeria tenella (E. tenella) is a species of Eimeria that causes haemorrhagic caecal coccidiosis, resulting in major economic losses in the global poultry industry. After E.

Dynamic expression of death receptor adapter proteins tradd and fadd in Eimeria tenella-induced host cell apoptosis.

The present study aimed to investigate the dynamic expression patterns of death-receptor adapter proteins TNF-receptor-associated death-domain protein (TRADD) and Fas-associated death-domain protein (FADD) in E. tenella-induced host-cell apoptosis. Culture techniques for primary chick embryo cecum epithelial cells, ELISA, hematoxylin-eosin staining, fluorescence quantitative PCR techniques, and Hoechst-Annexin V-PI apoptosis staining were used to detect the apoptosis rates and dynamic expression patterns of TRADD and FADD in E.

Dynamic changes in the main regulatory genes of mitochondrial permeability transition pore in Eimeria tenella host cells.

The purpose of the present study was to investigate the dynamic changes in the main regulatory genes of the mitochondrial permeability transition pore in E. tenella host cells. Primary chick embryo cecum epithelial cell culture techniques, spectrophotometer technology, Hoechst-Annexin V-PI apoptosis staining and ELISA were used to detect the apoptosis rate and dynamic changes of Bcl-2, Bcl-xl, Bax, Bak, Bid, Bad, HK-II, and ATP content in E.