Honey and pollen from and , stingless bees from northern Argentina, presented a particular microbiological profile and associated enzymatic activities. The cultured bacteria were mostly spp. (44%) and spp.
View Article and Find Full Text PDFBackground: American tegumentary leishmaniasis (ATL) is an endemic neglected tropical disease (NTD), its conventional treatment is toxic, slow, and invasive. Rapid diagnosis is crucial for the clinical management of suspected patients, so the development and use of low-cost, miniaturised and portable devices could be the key.
Objectives: This work aimed to develop a simple paper-based electrochemical platform for the serological detection of ATL.
From the first glucose biosensor from Updike and Hicks (1968), there was an explosion of research in biosensors for detecting a wide range of analytes [...
View Article and Find Full Text PDFSmart biosensors are becoming an important support for modern healthcare, even more so in the current context. Numerous smartphone-based biosensor developments were published in recent years, some highly effective and sensitive. However, when patents and patent applications related to smart biosensors for healthcare applications are analyzed, it is surprising to note that, after significant growth in the first half of the decade, the number of applications filed has decreased considerably in recent years.
View Article and Find Full Text PDFIn recent years, several devices have been developed for the direct measurement of hydrogen peroxide (H2O2), a key compound in biological processes and an important chemical reagent in industrial applications. Classical enzymatic biosensors for H2O2 have been recently outclassed by electrochemical sensors that take advantage of material properties in the nano range. Electrodes with metal nanoparticles (NPs) such as Pt, Au, Pd and Ag have been widely used, often in combination with organic and inorganic molecules to improve the sensing capabilities.
View Article and Find Full Text PDFWe present a low-cost, portable microfluidic platform that uses laminated polymethylmethacrylate chips, peristaltic micropumps and LEGO® Mindstorms components for the generation of magnetoliposomes that does not require extrusion steps. Mixtures of lipids reconstituted in ethanol and an aqueous phase were injected independently in order to generate a combination of laminar flows in such a way that we could effectively achieve four hydrodynamic focused nanovesicle generation streams. Monodisperse magnetoliposomes with characteristics comparable to those obtained by traditional methods have been obtained.
View Article and Find Full Text PDFGold-platinum nanowires are proposed as electrocatalysts for a real-time nonenzymatic impedancimetric detection of glucose. The electrochemical behavior of the obtained platform toward electrocatalytic oxidation of glucose, including a proposal for the detection mechanism, is shown.
View Article and Find Full Text PDFChrono-impedance technique (CIT) was implemented as a new transduction method for real time measurement of glucose in a biosensor system based in carbon paste (CP)/Ferrocene (FC)/glucose oxidase (GOx). The system presents high selectivity because the optimal stimulation signal composed by a 165mV DC potential and 50mV(RMS) AC signal at 0.4Hz was used.
View Article and Find Full Text PDFBio-recognition devices have captured special attention because they combine biological specificity and selectivity with electronics to perform environmental and biomedical analysis. Lindane is a recalcitrant pesticide considered potential carcinogen that has caused serious pollution problems. The purpose of the present study is to evaluate Streptomyces sp.
View Article and Find Full Text PDFThe chrono-impedance technique (CIT) for real time determination of glucose concentration in a first generation glucose oxidase/carbon paste electrode was implemented. The biosensor was polarized with a signal composed of 900 mV DC potential and 50 mV(RMS) AC signal at 0.4 Hz.
View Article and Find Full Text PDFBackground: In Impedance Microbiology, the time during which the measuring equipment is connected to the bipolar cells is rather long, usually between 6 to 24 hrs for microorganisms with duplication times in the order of less than one hour and concentrations ranging from 10(1) to 10(7) [CFU/ml]. Under these conditions, the electrode-electrolyte interface impedance may show a slow drift of about 2%/hr. By and large, growth curves superimposed on such drift do not stabilize, are less reproducible, and keep on distorting all over the measurement of the temporal reactive or resistive records due to interface changes, in turn originated in bacterial activity.
View Article and Find Full Text PDF