Extinction requires new RNA and protein synthesis and the soma of the cell right pedal dorsal 1 in Lymnaea stagnalis.

Lymnaea stagnalis were operantly conditioned to not perform aerial respiratory behavior. This learned response was subsequently extinguished. Here, we show that spaced extinction training is more effective than massed extinction training, in addition to the occurrence of spontaneous recovery.

Cooling blocks ITM and LTM formation and preserves memory.

In Lymnaea aerial respiratory behaviour can be operantly conditioned; snails learn not to perform this behaviour. Depending on the training procedure used, snails are competent to form either intermediate-term (ITM; lasting 1-3 h) or long-term (LTM; >4 h) memory. We found that cooling the snails for 1 h immediately after training was sufficient to block either ITM or LTM.