Characteristics of the human isoplanatic patch and implications for adaptive optics retinal imaging.

Conventional adaptive optics enables correction of high-order aberrations of the eye, but only for a single retinal point. When imaging extended regions of the retina, aberrations increase away from this point and degrade image quality. The zone over which aberrations do not change significantly is called the "isoplanatic patch.

Exploring ocular aberrations with a schematic human eye model.

Purpose: Advances in ophthalmic technologies now offer both the measurement and reduction of ocular aberrations by surgically or otherwise honing refraction in the anterior eye. Ocular aberrations, however, are known to change with a multitude of factors, including field position, accommodation level, and age. Thus, although static correction of aberrations provides some vision improvement, this may be less than expected.

Refraction and aberration across the horizontal central 10 degrees of the visual field.

Purpose: The purpose of this study was to measure refraction and aberrations across the horizontal central visual field.

Methods: Cycloplegic refraction was measured on eight subjects at 13 points across the horizontal central 10 degrees of the retina using a Hartmann-Shack wavefront sensor. Refractions were converted into mean sphere (M), 90 degrees to 180 degrees astigmatism (J180), and 45 degrees to 135 degrees astigmatism (J45) components.

Spectra and lifetimes of fluorescence resonance energy transfer fluorophores under two-photon excitation.

We show two-photon spectra and lifetimes acquired using conventional confocal microscopes equipped with an ultra-short pulsed laser and a time-gated intensified charge coupled device. We report on the two-photon spectra and lifetimes of Alexa350, enhanced green fluorescent protein (EGFP), EGFP-CD46, and Cy3 labelled antibodies. Cellular and extracellular EGFP two-photon spectra and lifetimes are compared.

Effect of ultrashort pulsed illumination on foci caused by a Fresnel zone plate.

The focal distribution produced by a zone plate under ultrashort pulsed laser illumination is investigated under the Fresnel approximation. A comparison of the diffraction patterns in the focal region between pulsed and continuous-wave illumination shows that the focal shape produced by a zone plate can be significantly altered when an ultrashort pulse is shorter than 100 fs. In particular, the focal width in the axial and the transverse directions is increased by approximately 5 and 85%, respectively,from continuous-wave illumination to 10-fs pulsed illumination.