Enzymatic method to measure β-1,3-β-1,6-glucan content in extracts and formulated products (GEM assay).

An enzymatic method to measure β-glucan content (GEM assay) is applicable in a variety of matrices. The method is composed of swelling the sample with KOH and initial digestion with a lyticase, which is followed by treatment with a mixture of exo-1,3-β-d-glucanase and β-glucosidase that converts the β-glucan to glucose. The glucose generated by the enzymatic hydrolysis is measured by another enzymatic method.