The Piauí-Real estuary is located along the southern coast of Sergipe state, Northeast Brazil. This estuary has great economic importance due to its physical, biological, and socioeconomic diversity, but it is subject to anthropogenic stress since the resident population in the town bordered by the estuarine system has grown in recent years. Thus, the possibility of sewage contamination originating from the approximately 450,000 inhabitants living within its drainage basin was investigated in this study.
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