[Molecular variability in geographically distant populations of Drosophila melanogaster at the Lim3 gene regulating nervous system development].

In this study, we compare the variability of the regulatory region of the Lim3 gene, which plays a key role in the development of the nervous system, in two populations of Drosophila melanogaster inhabiting the cities of Aleksandrov (Russia) and Raleigh (United States). The two population areas are located in different geographic regions and differ in their ecology. A comparison of nucleotide sequences of 16 (2010) and 20 (2011) alleles from the Alexandrov population showed that in both cases the variability level of the untranslated Lim3 region was considerably lower than that of the 5' regulatory region adjacent to the transcription start site.

[Evaluation of ovarian status in women who received anti-tumor therapy in childhood and adolescence].

The paper presents the results of a study of the ovarian reserve in young women who received treatment for malignant tumors in childhood and adolescence and are in complete clinical remission. The function of the reproductive system was evaluated by serum concentrations of gonadotropins, estradiol, anti-Müllerian hormone (AMH) and inhibin B. The results were compared to the treatment, patients' age at the beginning of therapy and at the time of the examination.

[Synthesis of asymmetric tetraarylporphyrins and its ytterbium complexes].

The synthesis of asymmetric meso-aryl-substituted porphyrins containing three 4-methoxycarbonylphenyl groups, and as a forth substituent 4-hydroxyphenyl or 4-hydroxy-3- methoxyphenyl radicals, or the isomeric 3- and 4-pyridyl substituents is described. O-alkyl derivatives of 4-hydroxyl residue are obtained. The ytterbium complexes ofthese porphyrins were synthesized and studied their luminescence spectral properties were studied.

[Neuroendocrine system in Drosophila melanogaster lifespan control].

Drosophila is used as a model organism to review the mechanisms of neuroendocrine system involvement in lifespan control. The role of neuron specific expression of genes participating in antioxidative system in lifespan control is described. Data on endocrine function of the nervous system in lifespan control are discussed.

Mitochondria-targeted plastoquinone derivatives as tools to interrupt execution of the aging program. 5. SkQ1 prolongs lifespan and prevents development of traits of senescence.

Very low (nano- and subnanomolar) concentrations of 10-(6'-plastoquinonyl) decyltriphenylphosphonium (SkQ1) were found to prolong lifespan of a fungus (Podospora anserina), a crustacean (Ceriodaphnia affinis), an insect (Drosophila melanogaster), and a mammal (mouse). In the latter case, median lifespan is doubled if animals live in a non-sterile vivarium. The lifespan increase is accompanied by rectangularization of the survival curves (an increase in survival is much larger at early than at late ages) and disappearance of typical traits of senescence or retardation of their development.

[Removing endosymbiotic Wolbachia specifically decreases lifespan of females and competitiveness in a laboratory strain of Drosophila melanogaster].

To understand specific symbiotic relationships ensuring stable existing of the bacterium Wolbachia in laboratory strains of Drosophila melanogaster, the imago lifespan and senescence rate, as well as competitiveness, have been evaluated as components of fitness in females from the following laboratory strains: (1) inbred strain 95 infected with Wolbachia; (2) two uninfected strains obtained by tetracycline treatment that were genetically similar to strain 95; and (3) two control, uninfected, wild-type laboratory strains that were used to assess the possible effects of the antibiotic on the studied characters in the absence of Wolbachia. The results have shown that infected females have longer lifespan and competitiveness than females with the same genotype uninfected with Wolbachia. The increase in the senescence and mortality rates with age was also slower in infected females.

[Genes regulating the development and functioning of the nervous system determine life span in Drosophila melanogaster].

Earlier, it has been shown that genes responsible for differences in longevity between wild-type Drosophila melanogaster lines 2b and Oregon are localized in region 7A6-B2, 36E4-37B9, 37B9-D2, and 64C-65C. Quantitative complementation tests were conducted between the gene mutations localized in these regions and involved in catecholamine biosynthesis (iav (inactive), Catsup (Catecholamines up), amd (alpha methyl dopa resistant), Dox-A2 (Diphenol oxidase A2), pie (pale)) and neuron development control (Fas3 (Fascyclin 3), tup (tail up), Lim3), on the one hand, and two different normal alleles of these genes in lines 2b and Oregon, on the other. Complementation was found for genes iav, Fas3, amd and ple.

[A transmaxillary transnasal approach to tumors of the base of the skull].

The microsurgical transmaxillary transnasal approach provides a wide access to the midline of the skull base. Since 1994 the approach was performed in 11 patients while resecting malignant and benign skull base tumors by involving the cribriform plate, orbit, maxillary and sphenoidal sinuses, nasal cavity, clivus, and cavernous sinus. The technique is suitable for extradural tumors of the skull base and can be combined with transcranial approaches for more extensive lesions.

[The results of microvascular decompression of the accessory nerve in patients with spastic torticollis].

The results of microvascular decompression of accessory nerve in patients with spastic torticollis are reviewed. 40 patients with spastic torticollis were treated. Unilateral microvascular decompression of the XIth cranial nerve were used in 11 cases, lateral in 23 cases.

[Effect of tyrosine kinase and tyrosine phosphatase inhibitors on ATP- and thapsigargin-induced CA2+ entry in rat peritoneal macrophages].

The effect of two structurally distinct tyrosine kinase inhibitors, genistein (100 microM) and methyl-2, 5-dihydroxycinnamate (25 microM) on ATP- and thapsigargin-induced Ca2+ signals in Fura-2-loaded rat peritoneal macrophages was investigated. Both compounds were shown to inhibit ATP-evoked Ca2+ entry but not to release from internal stores. Both compounds also inhibit the store-dependent or "capacitative" Ca2+ influx stimulated by emptying the intracellular Ca2+ stores with endoplasmic Ca(2+)-ATPase inhibitor thapsigargin (100 nM).

[A comparative study of fluoroquinolones and 3rd-generation cephalosporins in the prevention and treatment of experimental plague caused by Yersinia pestis strains typical and serologically atypical with respect to F1].

Fluoroquinolones (ciprofloxacin and pefloxacin) and 3rd generation cephalosporins (cefoperazone, cefotaxime, ceftazidime and ceftriaxone) were comparatively studied in the prevention and treatment of experimental plague in albino mice caused by F1+ and F1- strains of the plague microbe. Despite the phenotype of the strain which caused the infection, the drugs were highly efficient in the etiotropic therapy. However, in the experimental plague due to F1- strains it was needed to use the maximum mean daily doses of the fluoroquinolones, cefoperazone and cefotaxime.

[Surgical treatment of migrainous neuralgia].

The paper gives the results of surgical management in 18 patients with migrainous neuralgia who underwent 20 surgical interventions. High-frequency transcutaneous trigeminal rhizotomy eliminated pain in 2 of 6 patients. Microsurgical radicular exploration of the trigeminal nerve (1 case) and the intermediate facial nerve (2 cases) revealed no vascular compression of nerve fibers.

[The effect of docosatrienoic acid on the potassium channels of outward rectification in the membrane of rat peritoneal macrophages].

Application of docosatrienic acid was shown to dose-dependently decrease the peak K+ current amplitide and accelerate the potassium activation and inactivation kinetics at all membrane potentials. The data obtained suggests a direct effect of docosatrienic acid on the K+ channels in peritoneal macrophages.

[Cefoperazone in the prevention and treatment of experimental plague caused by typical and fraction-free pathogen strains in white mice].

The high susceptibility of the plague microbe to cefoperazone (MIC of 0.1-0.25 microgram/ml) did not depend on the causative agent ability to produce fraction I.

[Etiological factors in the trigeminal neuralgia syndrome].

Trigeminal neuralgia (TN) accompanies brain tumors, multiple sclerosis and vascular compression of the root of the trigeminal nerve. Among 80 TN examinees, multiple sclerosis, cerebellopontile angle tumors, compression of the root of the trigeminal nerve by arterial and venous vessels were diagnosed in 2.5 and 73 patients, respectively.

[Clinical aspects, pathogenesis and treatment of neurovascular spastic torticollis].

The authors describe the clinical picture and treatment of neurovascular spastic torticollis (NVST) with special reference to 20 patients with ST, who underwent microvascular decompression of the accessory nerves. Postural attenuation of ST served as a criterion for the screening of the patients. The authors describe the results of clinico-anatomic correlations, attesting to the diverse neurological picture of NVST and to the presence of correlations between the nystagmus in ST and compression of nerve XI of the posterior connective artery as well as between facial hyperkineses in ST patients and compression of the accessory nerves by the pathologically involuted vertebral and posteroinferior cerebellar arteries.

[The effect of N-bromoacetamide on the components of sodium channel inactivation in the membrane of Ranvier's node].

In voltage clamp experiments on the frog Ranvier node, the specific protein reagent, N-bromacetamide, significantly decelerates the sodium inactivation kinetics and makes it incomplete. After treatment with N-bromacetamide, both fast and slow inactivation time constants are increased and the proportion of inactivation components is changed favouring the slowly inactivating one in the wide range of membrane potentials. The results are consistent with a single channel population following the 3-state model of inactivation.

[The diagnostic effectiveness of erythrocyte immunoreagents made from different Pseudomonas aeruginosa antigens].

The diagnostic effectiveness of the passive hemagglutination (PHA) tests with the use of erythrocyte diagnostica (ED based on exotoxin A (ETA) and poly- and monovalent ED based on lipopolysaccharides (LPS) isolated from 5 most widespread P. aeruginosa serogroups was compared. 97 patients with different purulent septic diseases and 100 practically healthy adult donors were examined.

[Transfemoral lumbar epidural phlebography in the diagnosis of intervertebral disk hernia].

The diagnosis of herniated disks with the use of lumbar epidural phlebography is discussed. Fifty patients were examined. The diagnosis was verified during the operation in 38 of 44 patients.

[Effect of niflumic acid on the components of inactivation of the sodium channels of the Ranvier node membrane].

In voltage clamp experiments on the frog Ranvier node, niflumic acid did not alter fast or slow inactivation time courses in the wide range of membrane potentials but reduced the amplitude of the fast phase of inactivation. Fast and slow currents, corresponding respectively to fast and slow phases of inactivation, reversed at the same voltage, revealed different activation- and inactivation-voltage dependences both in intact fibre and after application of niflumic acid. The latter induced a shift of the steady-state inactivation curves for both components of inactivation towards more negative potentials without changing their steepness.

[Production of thermolabile enterotoxins of Escherichia coli by Yersinia enterocolitica and Yersinia pseudotuberculosis].

The plasmids pCG86 and RP4elt coding for thermolabile enterotoxins of Escherichia coli (LT) were transferred in conjugation to Yersinia enterocolitica and Yersinia pseudotuberculosis cells. Both plasmids were stably inherited by the recipient cells. The elt genes of the toxins were expressed in Yersinia cells at the level comparable to the one registered in Escherichia coli cells.