Repurposing the Veterinary Antibiotic Apramycin for Antibacterial and Antibiofilm Activity Against From Cystic Fibrosis Patients.

Objectives: To evaluate the antibacterial, antibiofilm, and antivirulence activities of apramycin, comparatively to tobramycin, against a set of from chronically infected cystic fibrosis (CF) patients.

Methods: The activity of antibiotics against planktonic cells was assessed by performing MIC, MBC, and time-kill assays. The activity against mature biofilms was evaluated, in a microtiter plate, both in terms of dispersion (crystal violet assay) and residual viability (viable cell count).