Penetration of beta-lactoglobulin into monoglyceride monolayers. dynamics, interactions, and topography of mixed films.

In this work we have analyzed the penetration of betalactoglobulin into a monoglyceride monolayer (monopalmitin or monoolein) spread at the air-water interface and its effects on the structural, dilatational, and topographical characteristics of mixed films. Dynamic tensiometry, surface film balance, Brewster angle microscopy (BAM), and surface dilatational rheology have been used, maintaining the temperature constant at 20 degrees C and the pH and ionic strength at 7 and 0.05 M, respectively.

Self-assembly of monoglycerides in beta-lactoglobulin adsorbed films at the air-water interface. Structural, topographical, and rheological consequences.

In this work, we have analyzed the structural, topographical, and surface dilatational characteristics of pure beta-lactoglobulin adsorbed films and the effect of the self-assembly of monoglycerides (monopalmitin or monoolein) in beta-lactoglobulin films at the air-water interface. Measurements were performed in a single device that incorporates a Wilhelmy-type film balance, Brewster angle microscopy, and interfacial dilatational rheology. The structural and topographical characteristics of beta-lactoglobulin adsorbed and spread films are similar.

Spreading of monoglycerides onto beta-casein adsorbed film. Structural and dilatational characteristics.

The effect of monoglycerides (monopalmitin and monoolein) on the structural, topographical, and dilatational characteristics of betacasein adsorbed film at the air-water interface has been analyzed by means of surface pressure (pi)-area (A) isotherms, Brewster angle microscopy (BAM), and surface dilatational rheology. The static and dynamic characteristics of the mixed films depend on the interfacial composition and the surface pressure. At surface pressures lower than that for the beta-casein collapse (at the equilibrium surface pressure of the protein, pi(e)(beta-casein)) a mixed film of beta-casein and monoglyceride may exist.

Thermodynamic and dynamic characteristics of monoglyceride monolayers penetrated by beta-casein.

In this work, we have analyzed the dynamics of the penetration of beta-casein into monoglyceride monolayers (monopalmitin and monoolein) and the structural, dilatational, and topographical characteristics of mixed films formed by monoglyceride penetrated by beta-casein. Different complementary experimental techniques [dynamic tensiometry, surface film balance, Brewster angle microscopy (BAM), and surface dilatational rheology] have been used, maintaining the temperature constant at 20 degrees C and the pH at 7. The surface pressure of the monoglyceride monolayer at the beginning of the penetration process (at pi(i)MP and pi(i)MO for monopalmitin and monoolein, respectively) was the variable studied.

The Effect of monoglycerides on structural and topographical characteristics of adsorbed beta-casein films at the air-water interface.

The effect of monoglycerides (monopalmitin and monoolein) on the structural and topographical characteristics of beta-casein adsorbed film at the air-water interface has been analyzed by means of surface pressure (pi)-area (A) isotherms and Brewster angle microscopy (BAM). At surface pressures lower than that for the beta-casein collapse (pi(c)(beta-casein)), attractive interactions between beta-casein and monoglycerides were observed. At higher surface pressures, the collapsed beta-casein is partially displaced from the interface by monoglycerides.