Enhancing Therapeutic Efficacy against Infection in a Murine Model Using Rifampicin-Loaded PLGA Nanoparticles.

The efficacy of rifampicin encapsulated in poly(lactic--glycolic acid) (PLGA) nanoparticles was evaluated for the treatment of BALB/c mice experimentally infected with . The PLGA nanoparticles loaded with rifampicin (RNP) were prepared using the single emulsification-solvent evaporation technique, resulting in nanoparticles with a hydrodynamic diameter of 138 ± 6 nm. The zeta potential and polydispersity index values indicated that the system was relatively stable with a narrow size distribution.

Spatiotemporal Temperature Distribution of NIR Irradiated Polypyrrole Nanoparticles and Effects of pH.

The spatiotemporal temperature distributions of NIR irradiated polypyrrole nanoparticles (PPN) were evaluated by varying PPN concentrations and the pH of suspensions. The PPN were synthesized by oxidative chemical polymerization, resulting in a hydrodynamic diameter of 98 ± 2 nm, which is maintained in the pH range of 4.2-10; while the zeta potential is significantly affected, decreasing from 20 ± 2 mV to -5 ± 1 mV at the same pH range.

PLGA nanoparticle preparations by emulsification and nanoprecipitation techniques: effects of formulation parameters.

This study presents the influence of the primary formulation parameters on the formation of poly-dl-lactic--glycolic nanoparticles by the emulsification-solvent evaporation, and the nanoprecipitation techniques. In the emulsification-solvent evaporation technique, the polymer and tensoactive concentrations, the organic solvent fraction, and the sonication amplitude effects were analyzed. Similarly, in the nanoprecipitation technique the polymer and tensoactive concentrations, the organic solvent fraction and the injection speed were varied.

Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation.

Background/aim: Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocarcinoma cells (H-441).

Materials And Methods: The reactive oxygen species (ROS) produced by the laser photoactivation of MB in aqueous solutions and cell cultures were measured with probes, and the cell viability was evaluated with a colorimetric assay.