Publications by authors named "Rosalina Guadarrama-Medina"
Article Synopsis
- Strong evidence suggests that fatty acids, not carbohydrates, are the primary energy source for Mycobacterium tuberculosis during infection and latency.
- The development of an in vitro model has shown that M. tuberculosis adapts to using long-chain fatty acids, leading to a shift in its metabolism and a slowed growth rate similar to dormant stages.
- This study highlights new roles for certain genes and tRNAs in drug tolerance, providing insights into latent tuberculosis and potentially revealing novel targets for treatment.
The study of the in vitro cell growth of mycobacteria still remains a fastidious, difficult, and time-consuming procedure. In addition, assessing mycobacterial growth in the laboratory is often complicated by cell aggregation and slow growth-rate. We now report that the use of a stainless steel spring in the culture led to an absence of large cell clumps, to a decrease of dead cells in the exponential phase and to growth of a more homogeneous population of large cells.
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