Publications by authors named "Rosa Maria Escobedo-Gracia-Medrano"

Air-dried leaves of a spp. AAB, cv. "Manzano" plant, known as in the Maya culture, were sequentially extracted with hexane, ethyl acetate, and methanol; the resulting extracts were investigated for their antimycobacterial activity against susceptible and drug-resistant strains of (MTB) using the Microplate Alamar Blue Assay.

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Article Synopsis
  • Somaclonal variation in regenerated plants can lead to DNA abnormalities such as aneuploidy and polyploidy.
  • Flow cytometry (FCM) utilizing propidium iodide is an effective method for assessing DNA content and ploidy variation in plant tissue cultures.
  • The study outlines a two-step method for preparing samples and analyzes DNA ploidy stability in banana plants regenerated from embryogenic cell suspensions.
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Most cultivated bananas (Musa spp.) are polyploids, and their fruits are seedless and propagated exclusively vegetatively; however, they can also be cloned by micropropagation techniques, viz., direct organogenesis (DO) or somatic embryogenesis (SE).

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The sequence-related amplified polymorphism (SRAP) technique, aimed for the amplification of open reading frames (ORFs), vis-â-vis that of the amplified fragment length polymorphisms (AFLP) were used to analyze the genetic variation and relationships among forty Musa accessions; which include commercial cultivars and wild species of interest for the genetic enhancement of Musa. A total of 403 SRAP and 837 AFLP amplicons were generated by 10 SRAP and 15 AFLP primer combinations, of which 353 and 787 bands were polymorphic, respectively. Both cluster analysis of unweighted pair-grouping method with arithmetic averages (UPGMA) and principal coordinate (PCO) analysis separated the forty accessions into their recognized sections (Eumusa, Australimusa, Callimusa and Rhodochlamys) and species.

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