Use of headspace solid-phase microextraction and headspace sorptive extraction for the detection of the volatile metabolites produced by toxigenic Fusarium species.

An efficient methodology was developed to determine the growth of toxigenic Fusarium spp., based on headspace solid-phase microextraction (SPME) and stir bar sorptive extraction of the fungal volatile metabolites produced. SPME and headspace sorptive extraction (HSSE) were used to monitor the de novo production of sesquiterpene hydrocarbons, such as trichodiene, a volatile marker and intermediate in the biosynthesis of trichothecenes.

Monitoring and fast detection of mycotoxin-producing fungi based on headspace solid-phase microextraction and headspace sorptive extraction of the volatile metabolites.

Solid phase microextraction in combination with capillary GC-MS was used as monitoring technique for the collection and detection of the fungal volatile metabolite (+)-aristolochene by sporulated surface cultures of Penicillium roqueforti. A comparison was made between different toxigenic and nontoxigenic strains of P. roqueforti.