Bone mineral density changes in lactating adolescent mothers during the first postpartum year.

Objectives: This study aimed to compare changes in whole body bone mineral density (wbBMD) during the first postpartum year in adolescent mothers with those of nulliparous adolescents.

Methods: The study included 21 adolescent mothers and 16 nulliparous adolescent non-indigenous Mexican women (State of Sonora) from a low income level. All mothers were assessed at 15 days (0.

Preparation, characterization, and process performance of composite fibrous adsorbents as cation exchangers for high throughput and high capacity bioseparations.

Fibrous materials are proposed as novel chromatographic supports depicting high throughput and high product capacity. In this work, a composite fiber harboring strong cation-exchange moieties has been investigated. Such materials were characterized by a plethora of physical methods including degree of swelling (DS), scanning electron microscope (SEM), confocal laser scanning microscopy (CLSM), and Fourier transform infrared spectroscopy-attenuated total reflection (FTIR-ATR).

Tailoring orthogonal proteomic routines to understand protein separation during ion exchange chromatography.

Surface charge, molecular weight, and folding state are known to influence protein chromatographic behaviour onto ion exchangers. Experimentally, information related to such factors can be gathered via 2-DE methods. The application of 2-D PAGE under denaturing/reducing conditions was already shown to reveal separation trends within a large protein population from cell extracts.

Assessing adsorbent-biomass interactions during expanded bed adsorption onto ion exchangers utilizing surface energetics.

Biomass adhesion onto an adsorbent matrix or "interaction" as well as biological particle co-adhesion or "aggregation" can severely affect the overall performance of many direct-contact methods for downstream processing of bioproducts. Studies to quantitatively describe this biomass-adsorbent interaction were developed utilizing surface energetics. An indirect thermodynamic approach via contact angle and zeta potential measurements was utilized.

Global screening of protein chromatographic behavior on ion exchangers from a complex cell proteome. Towards in silico downstream processing of bioproducts.

Protein separation during ion-exchange chromatography implies complex physicochemical events. This work has evaluated the chromatographic behaviour of a complex cell proteome on commercial agarose-based adsorbents. Various ligand types in the cation- and anion-exchange mode were studied.

High intake of saturated fat and early occurrence of specific biomarkers may explain the prevalence of chronic disease in northern Mexico.

To investigate whether the high prevalence of coronary heart disease (CHD) and type II diabetes prevalent in Northern Mexico could be related to the presence at a young age of biomarkers for chronic disease, 25 boys and 29 girls (8-12 y old) from a low socioeconomic group were recruited. Plasma lipids, LDL phenotype, apolipoproteins (apos), glucose, and insulin were evaluated. Analysis of 3-d dietary records indicated the typical intake of this region to be high in total fat (37-43% energy) and saturated fat (11-13% energy).

Dietary cholesterol does not increase biomarkers for chronic disease in a pediatric population from northern Mexico.

Background: An increased incidence of coronary artery disease (CAD) is prevalent in northern Mexico. Effects of specific dietary components on risk factors for CAD have not been evaluated in children.

Objective: The purpose was to evaluate the effects of dietary cholesterol provided by whole eggs on the lipoprotein profile, LDL size, and phenotype in children from this region.

Chemical extraction and direct adsorptive purification of recombinant human antigen Ro52.

The antigenic protein Ro52 was expressed in the E. coli system harboring a 6 x His tag in the form of insoluble inclusion bodies. Direct chemical extraction of the product using 6-8 M urea proved to be effective.