Impact of the LDL subfraction phenotype on Lp-PLA2 distribution, LDL modification and HDL composition in type 2 diabetes.

Background: Qualitative alterations of lipoproteins underlie the high incidence of atherosclerosis in diabetes. The objective of this study was to assess the impact of low-density lipoprotein (LDL) subfraction phenotype on the qualitative characteristics of LDL and high-density lipoprotein (HDL) in patients with type 2 diabetes.

Methods: One hundred twenty two patients with type 2 diabetes in poor glycemic control and 54 healthy subjects were included in the study.

A cause of falsely low HDL concentrations in HIV-infected patients: increased polyclonal serum immunoglobulin.

Objective: To describe the interference of polyclonal hypergammaglobulinemia in a direct HDL-c method.

Methods: HDL-c was measured by a direct method (Roche Diagnostics) in four HIV-infected patients showing polyclonal hypergammaglobulinemia and also by precipitation with phosphotungstic acid and ultracentrifugation at a density of 1.063 kg/L.