Selective Cytotoxicity of Kaempferia parviflora Extracts in Human Cell Lines.

Objective: Aims of this study were to (1) compare anti-proliferative activity between aqueous and ethanol Kaempferia parviflora (KP) extracts in both cancer (Human urinary bladder cancer cell, T24) and normal cell lines (Human umbilical vein endothelial cell, HUVEC). (2) confirm selective cytotoxicity of ethanol KP extract to normal and different cancer cell lines (3) investigate its cellular mechanism through p53 and SIRT1 gene expression.

Methods: Phytochemical difference between aqueous and ethanol extract was determined by thin layer chromatography (TLC).

Antioxidant Effect of Berberine and its Phenolic Derivatives Against Human Fibrosarcoma Cells.

Berberine (B1), isolated from stems of Coscinium fenestratum (Goetgh.) Colebr, was used as a principle structure to synthesize three phenolic derivatives: berberrubine (B2) with a single phenolic group, berberrubine chloride (B3) as a chloride counter ion derivative, and 2,3,9,10-tetra-hydroxyberberine chloride (B4) with four phenolic groups, to investigate their direct and indirect antioxidant activities. For DPPH assay, compounds B4, B3, and B2 showed good direct antioxidant activity (IC50 values=10.

Suppression of human fibrosarcoma cell metastasis by Phyllanthus emblica extract in vitro.

Phyllanthus emblica (PE) is known to exhibit various pharmacological properties. This study aimed to evaluate the antimetastatic potential of a PE aqueous extract. Cytotoxicity to human fibrosarcoma cells, HT1080, was determined by viability assay using the 3-(4,5-dimethylthiazol,2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent.

Antioxidant effects of gamma-oryzanol on human prostate cancer cells.

Background: To assess the antioxidant effects of gamma-oryzanol on human prostate cancer cells.

Materials And Methods: Cytotoxic activity of gamma-oryzanol on human DU145 and PC3 prostate cancer cells was determined by proliferation assay using 3-(4, 5-dimethylthiazol, 2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) reagent. mRNA levels of genes involved in the intracellular antioxidant system, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GSR) were determined by reverse transcription-polymerase chain reaction (RT-PCR).

Antimetastatic potential of N-acetylcysteine on human prostate cancer cells.

Objective: N-acetylcysteine (NAC), is one of the cheapest, safest and widely used over-the-counter-drugs in Thailand. Here the authors examine the antimetastatic potential of NAC on the metastasis of human prostate cancer cells.

Material And Method: Cytotoxicity of NAC to human prostate cancer cells, DU145 and PC3, were determined by proliferation assay using the 3-(4, 5-dimethylthiazol, 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) reagent.

Phytochemical and antioxidant studies of Laurera benguelensis growing in Thailand.

The aim of this study was to investigate metabolites of the lichen Laurera benguelensis. A high-performance liquid chromatographic (HPLC) method has been developed for the characterization of xanthones and anthraquinones in extracts of this lichen. Lichexanthone, secalonic acid D, norlichexanthon, parietin, emodin, teloschistin and citreorosein were detected in the lichen samples, which were collected from two places in Thailand.

Free radical scavenging and anti-acne activities of mangosteen fruit rind extracts prepared by different extraction methods.

The ethanol extracts of mangosteen fruit rinds prepared by several extraction methods were examined for their contents of bioactive compounds, DPPH-scavenging activity, and anti-acne producing bacteria against Propionibacterium acnes and Staphylococcus epidermidis. The dried powder of the fruit rind was extracted with 95% ethanol by maceration, percolation, Soxhlet extraction, ultrasonic extraction, and extraction using a magnetic stirrer. Soxhlet extraction promoted the maximum contents of crude extract (26.

N-acetylcysteine inhibits proliferation, adhesion, migration and invasion of human bladder cancer cells.

Objective: Bladder cancer is not only a major public health and economically burden for the patients but also a major clinical impact for Thai urologists. The authors' aim was to study the anti-metastatic effect of N-acetylcysteine (NAC), one of the cheap, safe and widely used over-the-counter-drugs in Thailand, on the human bladder cancer cells.

Material And Method: Effects of NAC at various concentrations on the growth, adhesion, migration, and invasion of the human bladder cancer cell line were assessed in vitro.

Comparison of bioactive compounds content, free radical scavenging and anti-acne inducing bacteria activities of extracts from the mangosteen fruit rind at two stages of maturity.

Contents of bioactive components, free radical scavenging and anti-acne producing bacteria activities of young and mature fruit rind extracts of mangosteen were compared. The young fruit rind extract contained significantly higher contents of phenolics and tannins and promoted higher free radical scavenging activity than the mature fruit rind extract, while the later extract contained higher contents of flavonoids and alpha-mangostin xanthone and gave higher anti-acne producing bacteria activity than the young fruit rind extract. Thus, the young and mature stages of mangosteen fruit rind should be beneficial for further development of antioxidant and anti-acne pharmaceutical preparations, respectively.

Comparison of free radical scavenging activity of Siamese neem tree (Azadirachta indica A. Juss var. siamensis Valeton) leaf extracts prepared by different methods of extraction.

Objective: The aim of this study was to investigate the antioxidant activity of the aqueous extracts of leaves of Siamese neem tree (Azadirachta indica A. Juss var. siamensis Valeton) from several extracting and drying methods using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging assay.

Antioxidant activity of Siamese neem tree (VP1209).

Leaves, fruits, flowers and stem bark extracts from the Siamese neem tree (Azadirachta indica A. Juss var. siamensis Valeton, Meliaceae) were assessed for antioxidant activity in vitro using the 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging assay, total antioxidant activity and inhibition of lipid peroxidation in Chago K1 cancer cell culture by the thiobarbituric acid reactive substances (TBARS) method.