Surface Reconstruction for Selective Oxidation of Tetrahydroisoquinoline.

Integration of hydrogen evolution with the oxidation of organic substances in one electrochemical system is highly desirable. However, achieving selective oxidation of organic substances in the integrated system is still highly challenging. In this study, a phosphorylated NiMoO nanoneedle-like array was designed as the catalytic active electrode for the integration of highly selective electrochemical dehydrogenation of tetrahydroisoquinolines (THIQs) with hydrogen production.

Effects of moxibustion with wheat-grain size cone at "Zusanli" (ST 36) on vascular injury and oxidative stress in high-fat diet rats through mTOR/HIF-1α/VEGF signaling pathway.

Objectives: To explore the effect mechanism of moxibustion with wheat-grain size cone at "Zusanli" (ST 36) on vascular injury and oxidative stress in hyperlipidemia through mammalian target of rapamycin (mTOR)/hypoxia inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF) signaling pathway.

Methods: Forty healthy male SD rats with SPF grade were randomly divided into a normal group, a model group, a moxibustion group, and an inhibitor group, with 10 rats in each one. The hyperlipidemia model was established by feeding a high-fat diet for 8 weeks in rats of the model group, the moxibustion group and the inhibitor group.

Characteristics of exopolysaccharides from Paecilomyces hepiali and their simulated digestion and fermentation in vitro by human intestinal microbiota.

The metabolic process of polysaccharides in gastrointestinal digestions and the effects of the resulting carbohydrates on the composition of gut microbes are important to explore their prebiotic properties. Therefore, the purpose of this study was to investigate the simulated digestion and fecal fermentation in vitro of three fractions (PHEPSs-1, PHEPSs-2 and PHEPSs-3) purified from the crude exopolysaccharides of Paecilomyces hepiali HN1 (PHEPSs) and to explore the potential prebiotic mechanisms. The three purified fractions were characterized by HPLC, UV, FT-IR, SEM and AFM, and they were all of galactoglucomannan family with molecular weight of 178, 232 and 119 kDa, respectively.

Nitrate Reduction by NiFe-LDH/CeO: Understanding the Synergistic Effect between Dual-Metal Sites and Dual Adsorption.

Electrocatalytic nitrate reduction reaction (EC-NITRR) shows a significant advantage for green reuse of the nitrate (NO) pollutant. However, the slow diffusion reaction limits the reaction rate in practical EC-NITRR, causing an unsatisfactory ammonia (NH) yield. In this work, a multifunctional NiFe-LDH/CeO with the dual adsorption effect (physisorption and chemisorption) and dual-metal sites (Ce and Fe) was fabricated by the electrodeposition method.

A novel immobilized horseradish peroxidase platform driven by visible light for the complete mineralization of sulfadiazine in water.

A novel immobilized enzyme driven by visible light was prepared and used for complete mineralization of antibiotics in water bodies. The immobilized enzyme was composed of carbon nitride modified by biochar (C/CN) and horseradish peroxidase (HRP), establishing the photo-enzyme coupling system with synergistic effect. Among them, the introduction of biochar not only improves the stability and loading capacity of the enzyme, but also improves the light absorption capacity and carrier separation efficiency of the photocatalyst.

Branched Porous NiN as a Catalytic Electrode for Selective Semidehydrogenation of Tetrahydroisoquinoline.

Oxygen evolution in electrochemical water splitting needs a high overpotential that significantly reduces the energy efficiency. To explore an alternative anodic reaction to promote the production of hydrogen at the other end of water splitting and at the same time to get high-value-added chemicals is highly desirable. Herein, we demonstrate a novel branched porous NiN catalyst that is prepared for dehydrogenation of tetrahydroisoquinoline, which acts as an anodic oxidation reaction to promote H formation on the other end.

Breeding of high-tolerance yeast by adaptive evolution and high-gravity brewing of mutant.

Background: Ethanol and osmotic stresses are the major limiting factors for brewing strong beer with high-gravity wort. Breeding of yeast strains with high osmotic and ethanol tolerance and studying very-high-gravity (VHG) brewing technology is of great significance for brewing strong beer.

Results: This study used an optimized microbial microdroplet culture (MMC) system for adaptive laboratory evolution (ALE) of Saccharomyces cerevisiae YN81 to improve its tolerance to osmotic and ethanol stress.

Gene cloning and molecular characterization of a thermostable chitosanase from Bacillus cereus TY24.

Background: An important conceptual advance in health and the environment has been recognized that enzymes play a key role in the green processing industries. Of particular interest, chitosanase is beneficial for recycling the chitosan resource and producing chitosan oligosaccharides. Also, chitosan gene expression and molecular characterization will promote understanding of the biological function of bacterial chitosanase as well as explore chitosanase for utilizing chitosan resources.

Upregulation of circFOXP1 attenuates inflammation and apoptosis induced by ox-LDL in human umbilical vein endothelial cells by regulating the miR-185-5p/BCL-2 axis.

The pathogenesis of coronary artery disease (CAD) is closely related to an abnormal function of the coronary arteries due to myocardial ischemia, hypoxia, or necrosis, which poses a threat to human health. Therefore, this study was conducted to evaluate the role of circFOXP1 in controlling endothelial cell function during atherosclerosis (AS), and further investigate its potential molecular mechanism of regulation. Through Starbase database analysis, we predicted that circFOXP1 can sponge miR-185-5p that targets BCL-2.

Production of surfactant-stable keratinase from Bacillus cereus YQ15 and its application as detergent additive.

Background: With the growing concern for the environment, there are trends that bio-utilization of keratinous waste by keratinases could ease the heavy burden of keratinous waste from the poultry processing and leather industry. Especially surfactant-stable keratinases are beneficial for the detergent industry. Therefore, the production of keratinase by Bacillus cereus YQ15 was improved; the characterization and use of keratinase in detergent were also studied.

Molecular Precursor Route to CuCoS Nanosheets: A High-Performance Pre-Catalyst for Oxygen Evolution and Its Application in Zn-Air Batteries.

Development of high-efficiency non-precious metal-based electrocatalysts to drive the complex four-electron process of the oxygen evolution reaction (OER) is crucial for production of hydrogen and energy storage components. Herein, bimetallic CuCoS nanosheets were created by a new molecular precursor route. The optimal CuCoS catalyst demonstrates superior performance to catalyze the OER with excellent stability, which was confirmed by the low overpotential of 290 mV at 10 mA cm in 1 M KOH.

Corrigendum: Acidic Alkaline Bacterial Degradation of Lignin Through Engineered Strain BL21(Lacc): Exploring the Differences in Chemical Structure, Morphology, and Degradation Products.

[This corrects the article DOI: 10.3389/fbioe.2020.

Acidic Alkaline Bacterial Degradation of Lignin Through Engineered Strain BL21(Lacc): Exploring the Differences in Chemical Structure, Morphology, and Degradation Products.

There is increasing interest in research on lignin biodegradation compounds as potential building blocks in applications related to renewable products. More attention is necessary to evaluate the effects of the initial pH conditions during the bacterial degradation of lignin. In this study we performed experiments on lignin biodegradation under acidic and mild alkaline conditions.

Recombinant expression and molecular engineering of the keratinase from Brevibacillus parabrevis for dehairing performance.

Keratinase is capable of distinctive degradation of keratin, which provides an eco-friendly approach for keratin waste management towards sustainable development. In this study, the recombinant keratinase (KERBP) from Brevibacillus parabrevis was successfully expressed in Escherichia coli. The purified KERBP had the specific activity of 6005.

A novel alkaline surfactant-stable keratinase with superior feather-degrading potential based on library screening strategy.

A novel keratinase was mined and expressed in Escherichia coli BL21 (DE3) via function-driven screening with fosmid library. The catalytic properties of purified keratinase were investigated in detail following enzyme purification. The recombinant keratinase was purified to homogeneity with an estimated molecular weight of 26kDa using nickel affinity chromatography, of which the optimal reaction pH and temperature were 10.

Biochemical characterization of a novel surfactant-stable serine keratinase with no collagenase activity from Brevibacillus parabrevis CGMCC 10798.

Dehairing is a high pollution process in leather industry. Conventionally, the lime-sulfide mediated chemical process for dehairing would lead to the discharge of pollutants and corrosion of industrial equipment. Concerning these problems, keratinase has become a promising candidate for dehairing process in recent years.

Biochemical Characterization of An Arginine-Specific Alkaline Trypsin from Bacillus licheniformis.

In the present study, we isolated a trypsin-producing strain DMN6 from the leather waste and identified it as Bacillus licheniformis through a two-step screening strategy. The trypsin activity was increased up to 140 from 20 U/mL through culture optimization. The enzyme was purified to electrophoretic homogeneity with a molecular mass of 44 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the specific activity of purified enzyme is 350 U/mg with Nα-Benzoyl-L-arginine ethylester as the substrate.

A metallo-keratinase from a newly isolated Acinetobacter sp. R-1 with low collagenase activity and its biotechnological application potential in leather industry.

Microbial keratinase is a well-recognized enzyme that can specifically degrade insoluble keratins. A keratinase-producing bacterium was isolated from a duck ranch soil and identified as Acinetobacter sp. R-1 based on the biochemical characteristics and 16S rDNA gene sequencing.

Small molecular amine mediated synthesis of hydrophilic CdS nanorods and their photoelectrochemical water splitting performance.

Photoelectrochemical (PEC) water splitting is a promising route for solar energy harvesting and storage, but it has been highly limited by the performance of the semiconductor photoelectrodes. Herein, we report a small molecular amine-mediated solvothermal method for synthesizing CdS nanorods. The obtained CdS nanorods are hydrophilic and can be easily dispersed in water.

Graphene-spindle shaped TiO₂ mesocrystal composites: facile synthesis and enhanced visible light photocatalytic performance.

Graphene (GR)-TiO2 mesocrystal composites were prepared by a facile template-free process based on the combination of sol-gel and solvothermal methods, and were characterized using field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), Raman spectroscopy, UV-vis diffuse reflectance spectroscopy (UV-vis DRS), nitrogen absorption and electron spin resonance (ESR). Visible light photocatalytic performance of GR-TiO2 composites was evaluated for photocatalytic degradation of organic dye Rhodamine B. It was found that the amount of graphene oxide (GO) added obviously affects morphologies of TiO2 mesocrystals and photocatalytic activities of as-prepared nanocomposites.

Removal of cefalexin using yeast surface-imprinted polymer prepared by atom transfer radical polymerization.

The first use of yeast as a support in the molecular imprinting field combined with atom transfer radical polymerization was described. Then, the as-prepared molecularly imprinted polymers were characterized by Fourier transmission infrared spectrometry, scanning electron microscope, thermogravimetric analysis, and elemental analysis. The obtained imprinted polymers demonstrated elliptical-shaped particles with the thickness of imprinting layer of 0.

Preparation of molecularly imprinted nanoparticles with superparamagnetic susceptibility through atom transfer radical emulsion polymerization for the selective recognition of tetracycline from aqueous medium.

In the work, we reported an effective method for the preparation of molecularly imprinted nanoparticles with superparamagnetic susceptibility through atom transfer radical emulsion polymerization (ATREP), and then as-prepared magnetic molecularly imprinted nanoparticles (MMINs) were evaluated as adsorbents for selective recognition of tetracycline (TC) molecules from aqueous medium. The resulting nanoparticles were characterized by FT-IR, TGA, VSM, SEM and TEM. The results demonstrated MMINs with a narrow diameter distribution were cross-linked with modified Fe3O4 particles, composed of imprinted layer and exhibited good magnetic sensitivity, magnetic and thermal stability.

A dimeric zinc(II) complex: bis-[μ-1,2-bis-(1,2,4-triazol-4-yl)ethane-κN:N]bis-[dinitritozinc(II)].

The coordination geometry of the Zn(II) atom in the title complex, [Zn(2)(NO(2))(4)(C(6)H(8)N(6))(2)], is distorted octa-hedral, in which the Zn(II) atom is coordinated by two N atoms from the triazole rings of two symmetry-related 1,2-bis-(1,2,4-triazol-4-yl)ethane ligands and four O atoms from two nitrite ligands. Two Zn(II) atoms are bridged by two organic ligands, forming a centrosymmetric dimer. Weak C-H⋯N and C-H⋯O hydrogen bonds play an important role in the inter-molecular packing.

Bis(μ-4-amino-3,5-dimethyl-4H-1,2,4-triazole)bis-[diiodidozinc(II)].

In the title compound, [Zn(2)I(4)(C(4)H(8)N(4))(2)], the Zn(II) atom is coordinated in a distorted tetra-hedral geometry by two N atoms from the triazole rings of two 4-amino-3,5-dimethyl-4H-1,2,4-triazole (admt) ligands and two iodide ligands. Doubly bridging admt ligands connect two Zn(II) atoms, forming a centrosymmetric dimer. Weak N-H⋯I and C-H⋯I hydrogen bonds play an important role in the inter-molecular packing.

Synthesis, characterization, bioactivities of copper complexes with N-allyl di(picolyl)amine.

Four copper(II) complexes with N-allyl di(picolyl)amine were synthesized and characterized by physico-chemical and spectroscopic methods. The spectrophotometric and fluorescence titration data indicate that the [(Aldpa)Cu(L)](ClO(4))(2) (L=dppz, dione, phen) with conjugated aromatic rings as coordinated ligands can be inserted into the base stacks of DNA more deeply than the [(Aldpa)CuCl(2)]. The copper(II) complexes [(Aldpa)Cu(L)](ClO(4))(2) (L=dppz, dione, phen) can inhibit the proliferation of the four cancer cells (Mcf-7, Eca-109, A549 and HeLa) with IC(50) 0.