DOT1L-mediated RAP80 methylation promotes BRCA1 recruitment to elicit DNA repair.

Breast Cancer Type 1 Susceptibility Protein (BRCA1) is a tumor-suppressor protein that regulates various cellular pathways, including those that are essential for preserving genome stability. One essential mechanism involves a BRCA1-A complex that is recruited to double-strand breaks (DSBs) by RAP80 before initiating DNA damage repair (DDR). How RAP80 itself is recruited to DNA damage sites, however, is unclear.

Structure-Based Discovery of Selective Histone Deacetylase 8 Degraders with Potent Anticancer Activity.

Inducing protein degradation by proteolysis targeting chimeras has gained tremendous momentum as a promising novel therapeutic strategy. Here, we report the design, synthesis, and biological characterization of highly potent proteolysis targeting chimeric small molecules targeting the epigenetic regulator histone deacetylase 8 (HDAC8). We developed potent and effective HDAC8 degraders, as exemplified by (), which effectively induced HDAC8 protein degradation and inhibited cancer cell growth even at low micromolar concentrations.

Yohimbine Ameliorates Temporomandibular Joint Chondrocyte Inflammation with Suppression of NF-κB Pathway.

Local joint inflammation plays an important role in the pathogenesis of temporomandibular joint (TMJ) osteoarthrosis (TMJOA). Yohimbine, an alpha-2 adrenergic receptor antagonist, possesses anti-inflammatory properties; however, the ability of Yohimbine to protect against TMJOA-associated chondrocyte inflammation remains unclear. We conducted in vitro and in vivo analyses to investigate whether Yohimbine could ameliorate TMJOA-induced chondrocyte inflammation and to elucidate the mechanisms involved.

Mandibular distraction osteogenesis in the treatment of pediatric temporomandibular joint ankylosis with micrognathia and obstructive sleep apnea syndrome: A case report with 4-year follow-up.

Temporomandibular joint (TMJ) ankylosis in pediatric patients is rare and may cause severe micrognathia and obstructive sleep apnea syndrome. The present study reports on the treatment and 4-year follow-up of a pediatric patient with early-onset bilateral TMJ ankylosis and severe secondary micrognathia, as well as obstructive sleep apnea syndrome. A typical 'bird face' appearance was noted with severe mandible retrognathism and a significant convex facial profile.

Role of osteoprotegerin in the regulation of dental epithelial‑mesenchymal signaling during tooth development.

Dental epithelial‑mesenchymal signaling is crucial for tooth development, but the detailed mechanism is not fully understood. Using microarray analysis, it was revealed that the expression of osteoprotegerin, an important factor regulating bone remodeling, significantly increased after removal of the dental epithelium. Immunohistochemical staining revealed that osteoprotegerin expression within the dental mesenchyme was quite low during the prenatal period, but significantly increased after birth.

Single implants in the esthetic zone: analysis of recent peri-implant soft tissue alterations and patient satisfaction. A photographic study.

Purpose: The appearance of the peri-implant soft tissue is a crucial factor in the success of implant therapy. However, no effective objective method exists to observe and document this factor over the long term. The aim of this clinical trial was to determine whether oral photographs can be used for peri-implant soft tissue data collection by observing peri-implant soft tissue alterations after placement of a single-tooth implant in the esthetic zone and to determine whether the pink esthetic score (PES) reflects patient satisfaction with the peri-implant soft tissue esthetic result.

Simultaneous maxillomandibular distraction osteogenesis in severe progressive hemifacial atrophy with two distractors.

Progressive hemifacial atrophy is a rare disorder characterized by an acquired, idiopathic, self-limited, unilateral facial atrophy involving skin, subcutaneous tissue, fat, muscle, and bone. Symmetry and contour restoration are the main treatment challenges. Among many techniques, microvascular reconstruction has been introduced as the gold standard to correct the atrophic deformity.

The study of distraction osteogenesis with nitinol shape memory alloy spring controlled by infrared light.

Objective: The objective of this study was to investigate the feasibility of distraction osteogenesis with a nitinol shape memory alloy spring controlled by infrared light.

Study Design: In each of 20 New Zealand white rabbits, a critical-size defect (15 x 10 mm) was made in the body of the mandible, and a 10 x 8-mm segmental osteotomy was performed just anterior to the defect to create a transporting disk. A heat-controlled distractor and a temperature detector were then inserted (experimental group).

Changes of mandibular movement tracings after the correction of mandibular protrusion by bilateral sagittal split ramus osteotomy.

Purpose: We investigated the effects on mandibular movement tracings after the correction of mandibular protrusion by bilateral sagittal split ramus osteotomy (BSSRO).

Patients And Methods: This study was comprised of 30 control subjects and 14 mandibular protrusion patients. Mandibular movements were recorded during opening, protrusion, and laterotrusion of the jaw with the ARCUSdigma 3-dimensional mandibular kinesiograph (KaVo Dental, Biberach, Germany).

Retinoic acid and sodium butyrate as cell cycle regulators in the treatment of oral squamous carcinoma cells.

All-trans retinoic acid (ATRA) and sodium butyrate (SB) have shown growth-inhibitory and differentiation-inducing properties to tumor cells when used as single agents or in combination, but the exact molecular mechanism still remains to be determined. In order to determine the mechanism of the synergy in treatment with RA and SB, we evaluated the growth inhibition capability of ATRA and SB, alone or in combination, in human oral squamous carcinoma cell lines SCC-1 and SCC-9, and identified the expression of cell cycle-related genes. ATRA and SB inhibited cell growth and induced cell cycle G1 arrest.

[Risk factors of fat necrosis in pectoralis major myocutaneous flaps].

Objective: To explore the risk factors of fat necrosis in pectoralis major myocutaneous flaps.

Methods: From May 1998 to December 2005, 82 patients underwent reconstruction of oral and maxillofacial defects with pectoralis major myocutaneous flaps in our hospital. Postoperative fat necrosis of the flaps was occurred in 10 cases.

[Correction of dento-maxillofacial deformities following cleft lip and palate repair with orthognathic surgery and orthodontics].

Objective: To restore good occlusion and face profile, the orthognathic operation and orthodontics were used to correct the dento-maxillofacial deformities following the repair of cleft lip and palate.

Methods: 21 patients (7 males and 14 females, mean age of 20.6 years) were included in this study.

[Maxilla reconstruction with the free iliac osteomuscular flap and simultaneous osseointegrated implant embeding].

Objective: To evaluate the clinical outcome of reconstruction of maxillary defects with vascularized iliac crest flap and simultaneous osseointegrated implant embedding.

Methods: During September to October 2003, two patients with maxillary defects from tumor resection underwent microsurgical reconstruction. The free iliac osteomuscular flap transferring and simultaneous osseointegrated implant embedding were performed to repair the defects.

[Expression of p15 and p16 proteins in tongue squamous cell carcinoma and their significances].

Background & Objective: Abnormal expression of p15 and p16 were commonly found in many kinds of primary tumors, but the possible correlation between p15 and p16 abnormalities and tongue neoplasms is still unknown. This study was designed to investigate the expression of p15 and p16 proteins and their possible correlation with clinicopathology and prognosis of tongue squamous cell carcinoma (TSCC).

Methods: The expression of p15 and p16 were detected by immunohistochemistry (ultra-sensitive SP method) and the results were analyzed quantitatively by imagine cytometry in 45 cases of TSCC and 10 cases of normal tongue tissues, thereafter, the results were analyzed with clinicopathological parameters and survival time.

[The effects of insulin-like growth factor-II on the proliferation of osteoblast-like cells in vitro].

Objective: To seek for the appropriate concentration, at which IGF-II can exerts its strong effects on postirradiation proliferation, physiological function and differentiation of the rat's osteoblast-like cells (ROB).

Methods: The osteoblast-like cells used were isolated from the calvariae of neonatal (one-day-old) SD rats by sequential enzymatic digestion. The third passages of the cells were irradiated with gamma-ray from a (60)Co source at the doses of 100, 400, 600, and 900 cGy.